Publicação
Fusion proteins as innovative hair cosmetic products
| Resumo: | Hair has an unquestionable importance in body image and in today's society. It is a complex system made up mostly of keratins. With the increasing demand for new hair care products, the cosmetic industry has focused on finding new alternatives to the chemical formulations currently used. Protein and protein-based formulations are seen as excellent alternatives. Among proteins, fusion proteins aroused particular interest and have recently been explored in the development of hair care cosmetic formulations. Protein expression is an essential step in the development of protein-based formulations. In this work, seven proteins based on the XZX protein (XZX–1, XZX–2, XZX–3, XZX–4, XZX–5, XZX–6 and XZX–7) were explored as new cosmetic agents. For the expression of XZX proteins (1 to 6) five culture conditions were tested. For the expression of XZX–7 protein, a screening was performed to determine the best commercial culture medium. Using DesignExpert® software, three optimizations were performed to find the best mixture of culture medium (commercial media, industrial by-products and additives) and the best pH and temperature conditions to express the XZX–7 protein. This protein was produced using the conditions previously optimized and characterized. The ability of XZX–7 to bind to virgin Asian hair and its potential for curling was evaluated. XZX proteins (2 to 6) were expressed in LB medium + IPTG for 24 hours at 37°C. However, an optimization of expressing conditions or a change in the expression host is necessary, in order to make the production of these proteins a process economical viable. After optimizing the culture medium and growth conditions determined by the experimental design, it was determined that the conditions for maximizing the expression of XZX-7 protein were: 10 g/L TB-AIM, 30g/L CSL, 0.934 g/ L NaCl, pH 7, 37°C. With this optimization, the protein yield for an OD600 = 0.1 was doubled from 0.0012 mg for the commercial TB-AIM medium to 0.0025 mg for the optimized medium. The XZX-7 protein demonstrated the ability to bind to Asian hair with a binding percentage of 17.15% in water and 33.03% in an ethanolic formulation. Regarding the curling capacity of XZX-7, the condition of 20 mg/mL in an ethanolic formulation with urea pre-treatment demonstrated the best curling efficiency after six shampoo washes. |
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| Autores principais: | Costa, André Filipe Fernandes |
| Assunto: | Experimental design Fusion proteins Hair Hair cosmetic products Cabelo Desenho experimental Produtos cosméticos para os cabelos Proteínas de fusão |
| Ano: | 2021 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| Resumo: | Hair has an unquestionable importance in body image and in today's society. It is a complex system made up mostly of keratins. With the increasing demand for new hair care products, the cosmetic industry has focused on finding new alternatives to the chemical formulations currently used. Protein and protein-based formulations are seen as excellent alternatives. Among proteins, fusion proteins aroused particular interest and have recently been explored in the development of hair care cosmetic formulations. Protein expression is an essential step in the development of protein-based formulations. In this work, seven proteins based on the XZX protein (XZX–1, XZX–2, XZX–3, XZX–4, XZX–5, XZX–6 and XZX–7) were explored as new cosmetic agents. For the expression of XZX proteins (1 to 6) five culture conditions were tested. For the expression of XZX–7 protein, a screening was performed to determine the best commercial culture medium. Using DesignExpert® software, three optimizations were performed to find the best mixture of culture medium (commercial media, industrial by-products and additives) and the best pH and temperature conditions to express the XZX–7 protein. This protein was produced using the conditions previously optimized and characterized. The ability of XZX–7 to bind to virgin Asian hair and its potential for curling was evaluated. XZX proteins (2 to 6) were expressed in LB medium + IPTG for 24 hours at 37°C. However, an optimization of expressing conditions or a change in the expression host is necessary, in order to make the production of these proteins a process economical viable. After optimizing the culture medium and growth conditions determined by the experimental design, it was determined that the conditions for maximizing the expression of XZX-7 protein were: 10 g/L TB-AIM, 30g/L CSL, 0.934 g/ L NaCl, pH 7, 37°C. With this optimization, the protein yield for an OD600 = 0.1 was doubled from 0.0012 mg for the commercial TB-AIM medium to 0.0025 mg for the optimized medium. The XZX-7 protein demonstrated the ability to bind to Asian hair with a binding percentage of 17.15% in water and 33.03% in an ethanolic formulation. Regarding the curling capacity of XZX-7, the condition of 20 mg/mL in an ethanolic formulation with urea pre-treatment demonstrated the best curling efficiency after six shampoo washes. |
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