Publicação
Characterisation of new aqueous two-phase systems using thermoseparating polymers
| Resumo: | Aqueous two-phase systems (ATPS) are increasingly being used for separation of proteins, cells and viruses and some small molecular weight substances since they offer a gentle and friendly environment. The use of new thermoseparating polymers opens new opportunities to develop more efficient aqueous two-phase systems as they make possible polymer recycling just by temperature control. Phase diagrams for Ucon-(NH4)2SO4 system at different temperatures shows that this system uses a smaller amount of salt and polymer than conventional systems, is thermoseparating and versatile, and particularly attractive as phase inversion is temperature dependent. For the system PEG8000-PVA10000 the results show, as expected, that the polymer concentration for phase formation is higher than for systems using PEG with higher molecular weights. |
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| Autores principais: | Pereira, Martinha |
| Outros Autores: | Madeira, Pedro; Wu, You-Ting; Teixeira, J. A.; Macedo, Eugénia |
| Ano: | 2001 |
| País: | Portugal |
| Tipo de documento: | comunicação em conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| Resumo: | Aqueous two-phase systems (ATPS) are increasingly being used for separation of proteins, cells and viruses and some small molecular weight substances since they offer a gentle and friendly environment. The use of new thermoseparating polymers opens new opportunities to develop more efficient aqueous two-phase systems as they make possible polymer recycling just by temperature control. Phase diagrams for Ucon-(NH4)2SO4 system at different temperatures shows that this system uses a smaller amount of salt and polymer than conventional systems, is thermoseparating and versatile, and particularly attractive as phase inversion is temperature dependent. For the system PEG8000-PVA10000 the results show, as expected, that the polymer concentration for phase formation is higher than for systems using PEG with higher molecular weights. |
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