Publicação
Selective cytotoxicity of Portuguese propolis ethyl acetate fraction towards renal cancer cells
| Resumo: | Renal cell carcinoma is the most lethal cancer of the urological system due to late diagnosis and treatment resistance. Propolis, a beehive product, is a valuable natural source of compounds with bioactivities and may be a beneficial addition to current anticancer treatments. A Portuguese propolis sample, its fractions (<i>n</i>-hexane, ethyl acetate, <i>n</i>-butanol and water) and three subfractions (<b>P1</b>–<b>P3</b>), were tested for their toxicity on A498, 786-O and Caki-2 renal cell carcinoma cell lines and the non-neoplastic HK2 kidney cells. The ethyl acetate fraction showed the strongest toxicity against A498 (IC<sub>50</sub> = 0.162 µg mL<sup>−1</sup>) and 786-O (IC<sub>50</sub> = 0.271 µg mL<sup>−1</sup>) cells. With similar toxicity against 786-O, <b>P1</b> (IC<sub>50</sub> = 3.8 µg mL<sup>−1</sup>) and <b>P3</b> (IC<sub>50</sub> = 3.1 µg mL<sup>−1</sup>) exhibited greater effect when combined (IC<sub>50</sub> = 2.5 µg mL<sup>−1</sup>). Results support the potential of propolis and its constituents as promising coadjuvants in renal cell carcinoma treatment. |
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| Autores principais: | Freitas, Ana Sofia |
| Outros Autores: | Costa, Marta Sílvia Freitas; Pontes, Olívia; Seidel, Veronique; Proença, M. Fernanda R. P.; Cardoso, Susana M.; Oliveira, Rui Pedro Soares de; Baltazar, Fátima; Almeida Aguiar, Cristina |
| Assunto: | Propolis Fractionation Phenolic compounds Pectolinarigenin Cytotoxic activity Renal cell carcinoma |
| Ano: | 2022 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| Resumo: | Renal cell carcinoma is the most lethal cancer of the urological system due to late diagnosis and treatment resistance. Propolis, a beehive product, is a valuable natural source of compounds with bioactivities and may be a beneficial addition to current anticancer treatments. A Portuguese propolis sample, its fractions (<i>n</i>-hexane, ethyl acetate, <i>n</i>-butanol and water) and three subfractions (<b>P1</b>–<b>P3</b>), were tested for their toxicity on A498, 786-O and Caki-2 renal cell carcinoma cell lines and the non-neoplastic HK2 kidney cells. The ethyl acetate fraction showed the strongest toxicity against A498 (IC<sub>50</sub> = 0.162 µg mL<sup>−1</sup>) and 786-O (IC<sub>50</sub> = 0.271 µg mL<sup>−1</sup>) cells. With similar toxicity against 786-O, <b>P1</b> (IC<sub>50</sub> = 3.8 µg mL<sup>−1</sup>) and <b>P3</b> (IC<sub>50</sub> = 3.1 µg mL<sup>−1</sup>) exhibited greater effect when combined (IC<sub>50</sub> = 2.5 µg mL<sup>−1</sup>). Results support the potential of propolis and its constituents as promising coadjuvants in renal cell carcinoma treatment. |
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