Publicação
Selective cytotoxicity of Portuguese propolis ethyl acetate fraction towards renal cancer cells
| Resumo: | Renal cell carcinoma is the most lethal cancer of the urological system due to late diagnosis and treatment resistance. Propolis, a beehive product, is a valuable natural source of compounds with bioactivities and may be a beneficial addition to current anticancer treatments. A Portuguese propolis sample, its fractions (<i>n</i>-hexane, ethyl acetate, <i>n</i>-butanol and water) and three subfractions (<b>P1</b>–<b>P3</b>), were tested for their toxicity on A498, 786-O and Caki-2 renal cell carcinoma cell lines and the non-neoplastic HK2 kidney cells. The ethyl acetate fraction showed the strongest toxicity against A498 (IC<sub>50</sub> = 0.162 µg mL<sup>−1</sup>) and 786-O (IC<sub>50</sub> = 0.271 µg mL<sup>−1</sup>) cells. With similar toxicity against 786-O, <b>P1</b> (IC<sub>50</sub> = 3.8 µg mL<sup>−1</sup>) and <b>P3</b> (IC<sub>50</sub> = 3.1 µg mL<sup>−1</sup>) exhibited greater effect when combined (IC<sub>50</sub> = 2.5 µg mL<sup>−1</sup>). Results support the potential of propolis and its constituents as promising coadjuvants in renal cell carcinoma treatment. |
|---|---|
| Autores principais: | Freitas, Ana Sofia |
| Outros Autores: | Costa, Marta Sílvia Freitas; Pontes, Olívia; Seidel, Veronique; Proença, M. Fernanda R. P.; Cardoso, Susana M.; Oliveira, Rui Pedro Soares de; Baltazar, Fátima; Almeida Aguiar, Cristina |
| Assunto: | Propolis Fractionation Phenolic compounds Pectolinarigenin Cytotoxic activity Renal cell carcinoma |
| Ano: | 2022 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1866271011946627072 |
|---|---|
| author | Freitas, Ana Sofia |
| author2 | Costa, Marta Sílvia Freitas Pontes, Olívia Seidel, Veronique Proença, M. Fernanda R. P. Cardoso, Susana M. Oliveira, Rui Pedro Soares de Baltazar, Fátima Almeida Aguiar, Cristina |
| author2_role | author author author author author author author author |
| author_facet | Freitas, Ana Sofia Costa, Marta Sílvia Freitas Pontes, Olívia Seidel, Veronique Proença, M. Fernanda R. P. Cardoso, Susana M. Oliveira, Rui Pedro Soares de Baltazar, Fátima Almeida Aguiar, Cristina |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Freitas, Ana Sofia\"},{\"Person.name\":\"Costa, Marta Sílvia Freitas\"},{\"Person.name\":\"Pontes, Olívia\"},{\"Person.name\":\"Seidel, Veronique\"},{\"Person.name\":\"Proença, M. Fernanda R. P.\"},{\"Person.name\":\"Cardoso, Susana M.\"},{\"Person.name\":\"Oliveira, Rui Pedro Soares de\"},{\"Person.name\":\"Baltazar, Fátima\"},{\"Person.name\":\"Almeida Aguiar, Cristina\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Freitas, Ana Sofia Costa, Marta Sílvia Freitas Pontes, Olívia Seidel, Veronique Proença, M. Fernanda R. P. Cardoso, Susana M. Oliveira, Rui Pedro Soares de Baltazar, Fátima Almeida Aguiar, Cristina |
| datacite.date.Accepted.fl_str_mv | 2022-06-22T00:00:00Z |
| datacite.date.available.fl_str_mv | 2022-10-24T08:45:09Z |
| datacite.date.embargoed.fl_str_mv | 2022-10-24T08:45:09Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Propolis Fractionation Phenolic compounds Pectolinarigenin Cytotoxic activity Renal cell carcinoma |
| datacite.titles.title.fl_str_mv | Selective cytotoxicity of Portuguese propolis ethyl acetate fraction towards renal cancer cells |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | Freitas, Ana Sofia Costa, Marta Sílvia Freitas Pontes, Olívia Seidel, Veronique Proença, M. Fernanda R. P. Cardoso, Susana M. Oliveira, Rui Pedro Soares de Baltazar, Fátima Almeida Aguiar, Cristina |
| dc.date.Accepted.fl_str_mv | 2022-06-22T00:00:00Z |
| dc.date.available.fl_str_mv | 2022-10-24T08:45:09Z |
| dc.date.embargoed.fl_str_mv | 2022-10-24T08:45:09Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/80295 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Multidisciplinary Digital Publishing Institute (MDPI) |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.rights.rights.copyright.fl_str_mv | openAccess |
| dc.subject.none.fl_str_mv | Propolis Fractionation Phenolic compounds Pectolinarigenin Cytotoxic activity Renal cell carcinoma |
| dc.title.fl_str_mv | Selective cytotoxicity of Portuguese propolis ethyl acetate fraction towards renal cancer cells |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Renal cell carcinoma is the most lethal cancer of the urological system due to late diagnosis and treatment resistance. Propolis, a beehive product, is a valuable natural source of compounds with bioactivities and may be a beneficial addition to current anticancer treatments. A Portuguese propolis sample, its fractions (<i>n</i>-hexane, ethyl acetate, <i>n</i>-butanol and water) and three subfractions (<b>P1</b>–<b>P3</b>), were tested for their toxicity on A498, 786-O and Caki-2 renal cell carcinoma cell lines and the non-neoplastic HK2 kidney cells. The ethyl acetate fraction showed the strongest toxicity against A498 (IC<sub>50</sub> = 0.162 µg mL<sup>−1</sup>) and 786-O (IC<sub>50</sub> = 0.271 µg mL<sup>−1</sup>) cells. With similar toxicity against 786-O, <b>P1</b> (IC<sub>50</sub> = 3.8 µg mL<sup>−1</sup>) and <b>P3</b> (IC<sub>50</sub> = 3.1 µg mL<sup>−1</sup>) exhibited greater effect when combined (IC<sub>50</sub> = 2.5 µg mL<sup>−1</sup>). Results support the potential of propolis and its constituents as promising coadjuvants in renal cell carcinoma treatment. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://prod-dspace.uminho.pt/bitstreams/521caf37-a191-49b6-834a-0161e793be0c/download |
| id | rum_af9406060985f04dd2fb9680ad452ea3 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/80295 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/80295 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Freitas, Ana Sofia Costa, Marta Sílvia Freitas Pontes, Olívia Seidel, Veronique Proença, M. Fernanda R. P. Cardoso, Susana M. Oliveira, Rui Pedro Soares de Baltazar, Fátima Almeida Aguiar, Cristina |
| publishDate | 2022 |
| publisher.none.fl_str_mv | Multidisciplinary Digital Publishing Institute (MDPI) |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engMultidisciplinary Digital Publishing Institute (MDPI)porRenal cell carcinoma is the most lethal cancer of the urological system due to late diagnosis and treatment resistance. Propolis, a beehive product, is a valuable natural source of compounds with bioactivities and may be a beneficial addition to current anticancer treatments. A Portuguese propolis sample, its fractions (<i>n</i>-hexane, ethyl acetate, <i>n</i>-butanol and water) and three subfractions (<b>P1</b>–<b>P3</b>), were tested for their toxicity on A498, 786-O and Caki-2 renal cell carcinoma cell lines and the non-neoplastic HK2 kidney cells. The ethyl acetate fraction showed the strongest toxicity against A498 (IC<sub>50</sub> = 0.162 µg mL<sup>−1</sup>) and 786-O (IC<sub>50</sub> = 0.271 µg mL<sup>−1</sup>) cells. With similar toxicity against 786-O, <b>P1</b> (IC<sub>50</sub> = 3.8 µg mL<sup>−1</sup>) and <b>P3</b> (IC<sub>50</sub> = 3.1 µg mL<sup>−1</sup>) exhibited greater effect when combined (IC<sub>50</sub> = 2.5 µg mL<sup>−1</sup>). Results support the potential of propolis and its constituents as promising coadjuvants in renal cell carcinoma treatment.application/pdfporSelective cytotoxicity of Portuguese propolis ethyl acetate fraction towards renal cancer cellsFreitas, Ana SofiaCosta, Marta Sílvia FreitasPontes, OlíviaSeidel, VeroniqueProença, M. Fernanda R. P.Cardoso, Susana M.Oliveira, Rui Pedro Soares deBaltazar, FátimaAlmeida Aguiar, CristinaHostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptDOIIsPartOf10.3390/molecules271340012022-10-24T08:45:09Z2022-06-222022-07-08T11:55:27Z2022-06-22T00:00:00ZHandlehttps://hdl.handle.net/1822/80295http://purl.org/coar/access_right/c_abf2open accessPropolisFractionationPhenolic compoundsPectolinarigeninCytotoxic activityRenal cell carcinoma1553549 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2022-06-22http://creativecommons.org/licenses/by/4.0/openAccesshttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/521caf37-a191-49b6-834a-0161e793be0c/download |
| spellingShingle | Selective cytotoxicity of Portuguese propolis ethyl acetate fraction towards renal cancer cells Freitas, Ana Sofia Propolis Fractionation Phenolic compounds Pectolinarigenin Cytotoxic activity Renal cell carcinoma |
| status | SINGLETON |
| subject.fl_str_mv | Propolis Fractionation Phenolic compounds Pectolinarigenin Cytotoxic activity Renal cell carcinoma |
| title | Selective cytotoxicity of Portuguese propolis ethyl acetate fraction towards renal cancer cells |
| title_full | Selective cytotoxicity of Portuguese propolis ethyl acetate fraction towards renal cancer cells |
| title_fullStr | Selective cytotoxicity of Portuguese propolis ethyl acetate fraction towards renal cancer cells |
| title_full_unstemmed | Selective cytotoxicity of Portuguese propolis ethyl acetate fraction towards renal cancer cells |
| title_short | Selective cytotoxicity of Portuguese propolis ethyl acetate fraction towards renal cancer cells |
| title_sort | Selective cytotoxicity of Portuguese propolis ethyl acetate fraction towards renal cancer cells |
| topic | Propolis Fractionation Phenolic compounds Pectolinarigenin Cytotoxic activity Renal cell carcinoma |
| topic_facet | Propolis Fractionation Phenolic compounds Pectolinarigenin Cytotoxic activity Renal cell carcinoma |
| url | https://hdl.handle.net/1822/80295 |
| visible | 1 |