Publicação
NAD(P)H–FAD fluorescence detection using multiphoton microscopy improved with highly selective optical filters
| Resumo: | Early detection of cancer is critical for improving patient survival rates, reducing morbidity and minimizing treatment costs. This study explores the use of multiphoton microscopy to enhance colorectal cancer detection, by using two-photon excitation fuorescence of intrinsic fuorophores. The diagnostic capability of this technique hinges on two key metabolic enzymes, nicotinamide adenine dinucleotide and favin adenine dinucleotide, which serve as intrinsic biomarkers of cellular metabolism. This study focuses on integrating two-photon excitation fuorescence (TPEF) into conventional colonoscopes by miniaturizing the optical setup and incorporating highly selective optical flters for nicotinamide adenine dinucleotide and favin adenine dinucleotide fuorescence detection. While the current work does not demonstrate a fully miniaturized implementation, the presented flters are explicitly designed for future miniaturization. Their thin-flm dielectric structure allows seamless integration into compact platforms, such as fber-optic probes or on-chip photodetectors, supporting the long-term objective of embedding TPEF into clinical colonoscopy systems. This paper introduces specially designed optical flters optimized for detecting nicotinamide adenine dinucleotide (at 485 nm) and favin adenine dinucleotide (at 535 nm) fuorescence signals. Our results demonstrate that these flters efectively distinguish fuorescence signals from nicotinamide adenine dinucleotide and favin adenine dinucleotide, enabling accurate diferentiation between normal and cancerous tissue phantoms. The redox ratio analysis demonstrated a clear diference between tumoral (0.9680±0.0307) and normal (0.6014±0.0035) phantoms, highlighting the potential of the developed flters for colorectal cancer detection. This advancement lays a strong foundation for non-invasive cancer diagnostics, potentially enhancing early detection and improving patient outcomes. |
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| Autores principais: | Freitas, Ruben Brandão |
| Outros Autores: | Rodrigues, Manuel José Lima Ferreira; Cunha, Nelssom F.; Pimenta, Sara; Belsley, Michael; Correia, J. H.; Maciel, Marino J. |
| Assunto: | Colorectal cancer detection Designed optical flters Intrinsic fuorophores Tissue phantoms Two-photon fuorescence |
| Ano: | 2025 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | português |
| Origem: | RepositóriUM - Universidade do Minho |
| Resumo: | Early detection of cancer is critical for improving patient survival rates, reducing morbidity and minimizing treatment costs. This study explores the use of multiphoton microscopy to enhance colorectal cancer detection, by using two-photon excitation fuorescence of intrinsic fuorophores. The diagnostic capability of this technique hinges on two key metabolic enzymes, nicotinamide adenine dinucleotide and favin adenine dinucleotide, which serve as intrinsic biomarkers of cellular metabolism. This study focuses on integrating two-photon excitation fuorescence (TPEF) into conventional colonoscopes by miniaturizing the optical setup and incorporating highly selective optical flters for nicotinamide adenine dinucleotide and favin adenine dinucleotide fuorescence detection. While the current work does not demonstrate a fully miniaturized implementation, the presented flters are explicitly designed for future miniaturization. Their thin-flm dielectric structure allows seamless integration into compact platforms, such as fber-optic probes or on-chip photodetectors, supporting the long-term objective of embedding TPEF into clinical colonoscopy systems. This paper introduces specially designed optical flters optimized for detecting nicotinamide adenine dinucleotide (at 485 nm) and favin adenine dinucleotide (at 535 nm) fuorescence signals. Our results demonstrate that these flters efectively distinguish fuorescence signals from nicotinamide adenine dinucleotide and favin adenine dinucleotide, enabling accurate diferentiation between normal and cancerous tissue phantoms. The redox ratio analysis demonstrated a clear diference between tumoral (0.9680±0.0307) and normal (0.6014±0.0035) phantoms, highlighting the potential of the developed flters for colorectal cancer detection. This advancement lays a strong foundation for non-invasive cancer diagnostics, potentially enhancing early detection and improving patient outcomes. |
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