Publicação
Purification of polysaccharides from a biofilm matrix by selective precipitation of proteins
| Resumo: | The objective was to obtain the polysaccharide fraction presented in the extracted polymeric matrix of a biofilm by differential precipitation of the protein content. This was tried by pH adjustment and by addition of trichloroacetic acid (TCA). The best yield of protein precipitation was with 20% (w/v) TCA, with more than 97% of the protein removed and without affecting the amount of polysaccharides in solution. |
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| Autores principais: | Oliveira, Rosário |
| Outros Autores: | Marques, F.; Azeredo, Joana |
| Assunto: | Differential precipitation Polysaccharides Proteins |
| Ano: | 1999 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| Resumo: | The objective was to obtain the polysaccharide fraction presented in the extracted polymeric matrix of a biofilm by differential precipitation of the protein content. This was tried by pH adjustment and by addition of trichloroacetic acid (TCA). The best yield of protein precipitation was with 20% (w/v) TCA, with more than 97% of the protein removed and without affecting the amount of polysaccharides in solution. |
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