Publicação
Enzymatic dyeing of keratinous materials
| Resumo: | This study reports on the dyeing of keratinous materials using appropriate enzymatic systems – laccases and protein disulfide isomerase. The enzymatic dyeing was performed as a batchwise process at the temperature and pH of maximum enzyme activity. Laccases generate the colour “in situ” starting from low molecular colourless compounds – dye precursor and dye modifiers. Different hues and depth of shades could be achieved varying the concentration of the modifiers and the time of laccase treatment. Protein disulfide isomerases, based on their ability to catalyze thiol-disufide exchange, including oxidation, reduction and rearrangement, were used for covalent fixation of novel cysteine-modified dyes on keratinous fibres. |
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| Autores principais: | Tzanov, Tzanko |
| Outros Autores: | Prada, Enrique; Silva, Carla J. S. M.; Zille, Andrea; Paulo, Artur Cavaco |
| Assunto: | Keratinous fibre Laccase Protein disulfide isomerase |
| Ano: | 2003 |
| País: | Portugal |
| Tipo de documento: | outro |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| Resumo: | This study reports on the dyeing of keratinous materials using appropriate enzymatic systems – laccases and protein disulfide isomerase. The enzymatic dyeing was performed as a batchwise process at the temperature and pH of maximum enzyme activity. Laccases generate the colour “in situ” starting from low molecular colourless compounds – dye precursor and dye modifiers. Different hues and depth of shades could be achieved varying the concentration of the modifiers and the time of laccase treatment. Protein disulfide isomerases, based on their ability to catalyze thiol-disufide exchange, including oxidation, reduction and rearrangement, were used for covalent fixation of novel cysteine-modified dyes on keratinous fibres. |
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