Publicação
Monocyte-derived macrophages and dermal fibroblasts-derived ECM making small talk
| Resumo: | [Excerpt] Introduction: 3D in vitro skin models allow studying molecular pathways that otherwise are only possible to explore using animal models. The incorporation of immune cells, such as macrophages, has been implemented to create immunocompetent analogues. Yet, the human biology representation in those models is limited, among other aspects, by the minimal extracellular matrix (ECM) mimicry[1]. In order to tackle this limitation, we have established a protocol to extract fibroblasts-own ECM. In this work, we hypothesised that those ECM extracts were not able to promote macrophage activation, which could compromise the physiologic phenotype of the model. [...] |
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| Autores principais: | Chaves, S. |
| Outros Autores: | Macedo, J.; Reis, Daniel Pedro Pires Alves; Silva, Lucília Pereira; Marques, A. P. |
| Assunto: | Immunity Immunomodulation macrophage skin |
| Ano: | 2023 |
| País: | Portugal |
| Tipo de documento: | outro |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| Resumo: | [Excerpt] Introduction: 3D in vitro skin models allow studying molecular pathways that otherwise are only possible to explore using animal models. The incorporation of immune cells, such as macrophages, has been implemented to create immunocompetent analogues. Yet, the human biology representation in those models is limited, among other aspects, by the minimal extracellular matrix (ECM) mimicry[1]. In order to tackle this limitation, we have established a protocol to extract fibroblasts-own ECM. In this work, we hypothesised that those ECM extracts were not able to promote macrophage activation, which could compromise the physiologic phenotype of the model. [...] |
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