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Deciphering the impact of activated carbon, magnetite and zeolite on the activity of syntrophic cultures and pure cultures of methanogens

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Detalhes bibliográficos
Resumo:Biomethane has a paramount role in the emergent energy transition occurring in Europe, and biotechnological strategies to accelerate biomethane production are needed. In this work, we investigated the effect of several materials (activated carbon (AC), magnetite and zeolite) on the activity of methanogenic cultures, including pure cultures and complex microbial communities. Anaerobic incubations were set up with and without materials and the effects on microbial activity were monitored by following methane production and investigating gene expression. Overall, the presence of materials had a positive effect with few exceptions. Nevertheless, we could not find a material with a broad-spectrum capacity to enhance methane production rate by all cultures tested. Metatranscriptomics analysis revealed no changes of typical syntrophic relationships in the presence of AC, as Syntrophomonadaceae microorganisms were the ones expressing the majority of the genes involved in butyrate degradation and hydrogen production, and hydrogenotrophic methanogens were highly abundant.
Autores principais:Braga, Cátia Sofia Neves
Outros Autores:Martins, Gilberto; Duarte, Maria Salomé Lira; Costa, João Carlos Sequeira; Soares, O. S. G. P.; Pereira, M. F. R.; Pereira, I. C.; Alves, M. M.; Pereira, Luciana; Salvador, Andreia Filipa Ferreira
Assunto:Methane Materials Anaerobic cultures
Ano:2024
País:Portugal
Tipo de documento:comunicação em conferência
Tipo de acesso:acesso aberto
Instituição associada:Universidade do Minho
Idioma:inglês
Origem:RepositóriUM - Universidade do Minho
Descrição
Resumo:Biomethane has a paramount role in the emergent energy transition occurring in Europe, and biotechnological strategies to accelerate biomethane production are needed. In this work, we investigated the effect of several materials (activated carbon (AC), magnetite and zeolite) on the activity of methanogenic cultures, including pure cultures and complex microbial communities. Anaerobic incubations were set up with and without materials and the effects on microbial activity were monitored by following methane production and investigating gene expression. Overall, the presence of materials had a positive effect with few exceptions. Nevertheless, we could not find a material with a broad-spectrum capacity to enhance methane production rate by all cultures tested. Metatranscriptomics analysis revealed no changes of typical syntrophic relationships in the presence of AC, as Syntrophomonadaceae microorganisms were the ones expressing the majority of the genes involved in butyrate degradation and hydrogen production, and hydrogenotrophic methanogens were highly abundant.