Publicação
A new method for yeast phagocytosis analysis by flow cytometry
| Resumo: | Herein we developed a method based on the quenching effect of propidium iodide over Sytox-Green fluorescence to assess yeast phagocytosis by flow cytometry. It allows accurate quantification of living from dead phagocytes; internalized from non-internalized cells, maintaining yeast fluorescence within phagocytes; and the different associations between phagocytes and fungal cells. (C) 2014 Elsevier B.V. All rights reserved. |
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| Autores principais: | Carneiro, Maria Catarina Santos |
| Outros Autores: | Vaz, Catarina Oliveira; Pais, Célia; Sampaio, Paula; Pereira, Joana Isabel Carvalho |
| Assunto: | Candida spp Flow cytometry Sytox green Propidium iodide Phagocytosis PAMPs |
| Ano: | 2014 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| Resumo: | Herein we developed a method based on the quenching effect of propidium iodide over Sytox-Green fluorescence to assess yeast phagocytosis by flow cytometry. It allows accurate quantification of living from dead phagocytes; internalized from non-internalized cells, maintaining yeast fluorescence within phagocytes; and the different associations between phagocytes and fungal cells. (C) 2014 Elsevier B.V. All rights reserved. |
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