Publicação
Influence of the lens material in daily CLs incorporating drug-loaded Chitosan/Hyaluronic Acid nanoparticles
| Resumo: | Purpose: To investigate the behavior of different contact lens materials with incorporated Chitosan/Hyaluronic Acid (Ch/HA) nanoparticles loaded with the anticancer drug methotrexate (MTX). Methods: MTX-loaded nanoparticles were characterized with respect to their size and zeta potential (by Dynamic Light Scattering), stability, drug encapsulation efficiency, drug loading capacity and release profile (using UV/visible absorption and fluorescence spectroscopies). Two different materials of silicone-hydrogel daily contact lenses (CLs) were investigated: Senofilcon A and Delefilcon A. The contact lenses were immersed in the solution of drug-laden Ch/HA nanoparticles for two days at a temperature of 4ºC. Before and after this procedure, the CLs’ refractive index and water content were measured with the refractometer CLR-1270 (Index Instruments) and UV/visible transmittance was determined with the Shimadzu UV-VIS-NIR (model UV-360 Plus). Release tests were carried out in a pH 7.4 buffer solution at room temperature (25±1ºC) under agitation of 380 rpm. Results: The obtained nanoparticles (NPs) have a negatively charged surface (-10 mV), and hydrodynamic diameters of 284 ± 16 nm (at pH=7.4) and stability for at least one month at 4 ºC. The NPs exhibit a drug encapsulation efficiency of 98 ± 0.5% and a loading capacity of 64 ± 0.3%. The NPs adsorption efficiency to silicone-hydrogel CLs was 96 ± 0.7% (corresponding to 19±0.14 mg of NPs). The release of MTX from the CLs showed a sustained release profile. Senofilcon A lenses released 33 ± 5% and Delefilcon A lenses released 26 ± 4% of MTX content, after 24 hours. No variations in CLs’ refractive index and water content were detected after immersion (p>0.05) and UV/visible transmittance was always above 85%. Conclusion: The Ch/HA nanoparticles are suitable for a sustained release of the anticancer drug MTX from daily silicone-hydrogel CLs, with Senofilcon A presenting a higher release capability. |
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| Autores principais: | Vale, Sofia |
| Outros Autores: | Veloso, Sérgio Rafael Silva; Lira, Madalena; Castanheira, Elisabete M. S. |
| Assunto: | Chitosan Hyaluronic Acid Contact lens Nanoparticles |
| Ano: | 2024 |
| País: | Portugal |
| Tipo de documento: | outro |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| Resumo: | Purpose: To investigate the behavior of different contact lens materials with incorporated Chitosan/Hyaluronic Acid (Ch/HA) nanoparticles loaded with the anticancer drug methotrexate (MTX). Methods: MTX-loaded nanoparticles were characterized with respect to their size and zeta potential (by Dynamic Light Scattering), stability, drug encapsulation efficiency, drug loading capacity and release profile (using UV/visible absorption and fluorescence spectroscopies). Two different materials of silicone-hydrogel daily contact lenses (CLs) were investigated: Senofilcon A and Delefilcon A. The contact lenses were immersed in the solution of drug-laden Ch/HA nanoparticles for two days at a temperature of 4ºC. Before and after this procedure, the CLs’ refractive index and water content were measured with the refractometer CLR-1270 (Index Instruments) and UV/visible transmittance was determined with the Shimadzu UV-VIS-NIR (model UV-360 Plus). Release tests were carried out in a pH 7.4 buffer solution at room temperature (25±1ºC) under agitation of 380 rpm. Results: The obtained nanoparticles (NPs) have a negatively charged surface (-10 mV), and hydrodynamic diameters of 284 ± 16 nm (at pH=7.4) and stability for at least one month at 4 ºC. The NPs exhibit a drug encapsulation efficiency of 98 ± 0.5% and a loading capacity of 64 ± 0.3%. The NPs adsorption efficiency to silicone-hydrogel CLs was 96 ± 0.7% (corresponding to 19±0.14 mg of NPs). The release of MTX from the CLs showed a sustained release profile. Senofilcon A lenses released 33 ± 5% and Delefilcon A lenses released 26 ± 4% of MTX content, after 24 hours. No variations in CLs’ refractive index and water content were detected after immersion (p>0.05) and UV/visible transmittance was always above 85%. Conclusion: The Ch/HA nanoparticles are suitable for a sustained release of the anticancer drug MTX from daily silicone-hydrogel CLs, with Senofilcon A presenting a higher release capability. |
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