Publicação
Evaluation of dietary carbohydrate and rosmarinic acid on intestinal expression of glucose transporters and digestive enzymes
| Resumo: | The impact of dietary habits on human health is well known and diet plays a significant role in chronic and age related diseases. This study had the following objectives: (1) to characterize the effects of different types of dietary carbohydrate on the expression of the digestive enzyme sucrose-isomaltase (SI) and of the glucose transporters SGLT1 and GLUT2 (sodium dependent transporter and facilitated diffusion, respectively) in intestinal epithelial cells, and (2) describe the effects of the phytochemical agent RA on the modulation of these processes. Carbohydrate digestion and absorption by the intestine determine the rise in glucose levels of the blood after a meal and in diabetic patients these processes are increased which contributes to the hyperglycemia that characterizes the disease. Other dietary constituents, namely phytochemicals, may have a beneficial effect in this scenario by decreasing carbohydrate digestion and/or intestinal glucose uptake. This study shows that when carbohydrates are removed from the diet there is a marked decrease in SI, SGLT1 and GLUT2 not only at the apical membrane of enterocytes but also in enterocyte whole cell homogenates. Upon reintroduction of diets rich in carbohydrate (glucose or amylopectin) for 7 or 14 days, there is a marked increase in the enterocyte expression of the three proteins. The effects are more pronounced in the glucose diet which suggests a decisive role for luminal glucose on the adaptive response to increasing dietary carbohydrates. The phytochemical RA demonstrated to decrease the extent of the adaptive increase in the digestive enzyme as well as in the glucose transporters revealing its potential as a regulator of intestinal glucose absorption. This work is collaboration with Dr. Simon Lee of the University of Macau, China. |
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| Autores principais: | Vu Anh Tran |
| Ano: | 2012 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| Resumo: | The impact of dietary habits on human health is well known and diet plays a significant role in chronic and age related diseases. This study had the following objectives: (1) to characterize the effects of different types of dietary carbohydrate on the expression of the digestive enzyme sucrose-isomaltase (SI) and of the glucose transporters SGLT1 and GLUT2 (sodium dependent transporter and facilitated diffusion, respectively) in intestinal epithelial cells, and (2) describe the effects of the phytochemical agent RA on the modulation of these processes. Carbohydrate digestion and absorption by the intestine determine the rise in glucose levels of the blood after a meal and in diabetic patients these processes are increased which contributes to the hyperglycemia that characterizes the disease. Other dietary constituents, namely phytochemicals, may have a beneficial effect in this scenario by decreasing carbohydrate digestion and/or intestinal glucose uptake. This study shows that when carbohydrates are removed from the diet there is a marked decrease in SI, SGLT1 and GLUT2 not only at the apical membrane of enterocytes but also in enterocyte whole cell homogenates. Upon reintroduction of diets rich in carbohydrate (glucose or amylopectin) for 7 or 14 days, there is a marked increase in the enterocyte expression of the three proteins. The effects are more pronounced in the glucose diet which suggests a decisive role for luminal glucose on the adaptive response to increasing dietary carbohydrates. The phytochemical RA demonstrated to decrease the extent of the adaptive increase in the digestive enzyme as well as in the glucose transporters revealing its potential as a regulator of intestinal glucose absorption. This work is collaboration with Dr. Simon Lee of the University of Macau, China. |
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