Publicação
Desenvolvimento de polímeros recombinantes antimicrobianos para regeneração de feridas
| Resumo: | Skin wound infections pose a significant challenge, and the growing threat of antibiotic-resistant infections requires the development of new materials with effective antimicrobial properties that can simultaneously prevent bacterial proliferation and promote healing. Recombinant DNA technology enables the construction of recombinant protein polymers, that can be expressed in microbial cell factories, giving rise to materials with personalized chemical, physical and biological properties. These include Silk-elastin-like-proteins (SELPs), which combine in the same molecule the tensile strength of silk and the resilience and elasticity of elastin and are recognized for their biocompatibility and mechanical properties. As they are fully customizable, it is possible to modify their sequence to introduce bioactive domains, such as antimicrobial peptides. These small molecules have emerged as a promising solution due to their ability to fight a wide variety of microorganisms. In this work, functionalized polymers were developed by adding the sequence of antimicrobial peptides to the SELP-59A polymer: BMAP18, LL37 and Indolicidin, with the introduction of a 10-glycine spacer to give peptides greater conformational freedom. These polymers were produced in E. coli, purified using non-chromatographic methods and processed into films. The functionalized films showed high antimicrobial activity, completely inhibiting the growth of the strains tested, possibly due to the greater conformational freedom conferred by the spacer. In addition, the films were non-hemolytic and biocompatible, with a slight proliferative effect on HaCAT cells after 48 h of incubation. The physicochemical characterization of the films showed a hydrophilic surface, a high degree of swelling with rapid stabilization, and a slight degradation after 42 days. These results highlight the potential of the films developed for biomedical applications, combining favorable physicochemical properties, antimicrobial efficacy and biological safety. |
|---|---|
| Autores principais: | Silva, Beatriz Isabel da Cunha e |
| Assunto: | Antimicrobial peptide Film Recombinant protein polymer SELP Filme Péptido antimicrobiano Polímero proteico recombinante |
| Ano: | 2024 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | português |
| Origem: | RepositóriUM - Universidade do Minho |
| Resumo: | Skin wound infections pose a significant challenge, and the growing threat of antibiotic-resistant infections requires the development of new materials with effective antimicrobial properties that can simultaneously prevent bacterial proliferation and promote healing. Recombinant DNA technology enables the construction of recombinant protein polymers, that can be expressed in microbial cell factories, giving rise to materials with personalized chemical, physical and biological properties. These include Silk-elastin-like-proteins (SELPs), which combine in the same molecule the tensile strength of silk and the resilience and elasticity of elastin and are recognized for their biocompatibility and mechanical properties. As they are fully customizable, it is possible to modify their sequence to introduce bioactive domains, such as antimicrobial peptides. These small molecules have emerged as a promising solution due to their ability to fight a wide variety of microorganisms. In this work, functionalized polymers were developed by adding the sequence of antimicrobial peptides to the SELP-59A polymer: BMAP18, LL37 and Indolicidin, with the introduction of a 10-glycine spacer to give peptides greater conformational freedom. These polymers were produced in E. coli, purified using non-chromatographic methods and processed into films. The functionalized films showed high antimicrobial activity, completely inhibiting the growth of the strains tested, possibly due to the greater conformational freedom conferred by the spacer. In addition, the films were non-hemolytic and biocompatible, with a slight proliferative effect on HaCAT cells after 48 h of incubation. The physicochemical characterization of the films showed a hydrophilic surface, a high degree of swelling with rapid stabilization, and a slight degradation after 42 days. These results highlight the potential of the films developed for biomedical applications, combining favorable physicochemical properties, antimicrobial efficacy and biological safety. |
|---|