Publicação
Characterization of cetacean microbiomes as a health assessment landmark
| Resumo: | The dolphins are key animal groups acting as sentinels of disturbances of marine environments, reflecting health and status of lower trophic levels. Bacterial communities associated with these organisms (the microbiome) play important roles in maintaining health and causing disease. Knowing that only a few percentages of all microorganisms observed in nature can be cultured under conventional laboratory conditions, with the rise of next-generation sequencing technologies it became possible to access to the diversity and genetics of symbiotic microbes naturally residing into their hosts, the dolphins, by separation of exclusive nucleic acid species, the V3-V4 region of the 16S rRNA gene. 10 samples, from the stomach, intestine, oral and genital mucosa, the duodenal bulb, tongue, blowhole, skin lesion, oral cavity and from the colon-fluid, were obtained from the dolphin 1, a striped dolphin from specie Stenella coeruleoalba, were sequenced in Illumina MiSeq platform, exhibiting good quality scores for all bases and sequence quality. The analysis of the microbial diversity in Mothur and MG-RAST allowed concluding that the samples from the stomach, swab and tissue, are mainly constituted by bacterial species from the family Mycoplasmataceae. By his turn the intestine swab samples is mostly colonized by the Vibrionaceae, family of Proteobacteria. The dolphin’s genital mucosa is colonized by several microorganisms equally distributed including Fusobacteriaceae and Leptotrichiaceae and Pasteurellaceae families. On the oral mucosa and tongue, once again is largely present the Pasteurellaceae family of bacteria, but also the Campylobacter family. The duodenal bulb is practically all colonized by the Peptostreptococcaceae bacteria, whereas the Colon fluid is constituted by the Pasteurellaceae family and Vibrionaceae family. By his turn, the blowhole sample is comprised mainly by Pasteurellaceae, while the skin lesion sample is colonized mostly by Cardiobacteriaceae and other marine microorganisms. With this work, sampling methodologies and sample processing conditions were established, which proved to be quite reproducible once replicates for each sample type were fairly consistent. |
|---|---|
| Autores principais: | Araújo, Ricardo Manuel Pinto |
| Ano: | 2014 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| Resumo: | The dolphins are key animal groups acting as sentinels of disturbances of marine environments, reflecting health and status of lower trophic levels. Bacterial communities associated with these organisms (the microbiome) play important roles in maintaining health and causing disease. Knowing that only a few percentages of all microorganisms observed in nature can be cultured under conventional laboratory conditions, with the rise of next-generation sequencing technologies it became possible to access to the diversity and genetics of symbiotic microbes naturally residing into their hosts, the dolphins, by separation of exclusive nucleic acid species, the V3-V4 region of the 16S rRNA gene. 10 samples, from the stomach, intestine, oral and genital mucosa, the duodenal bulb, tongue, blowhole, skin lesion, oral cavity and from the colon-fluid, were obtained from the dolphin 1, a striped dolphin from specie Stenella coeruleoalba, were sequenced in Illumina MiSeq platform, exhibiting good quality scores for all bases and sequence quality. The analysis of the microbial diversity in Mothur and MG-RAST allowed concluding that the samples from the stomach, swab and tissue, are mainly constituted by bacterial species from the family Mycoplasmataceae. By his turn the intestine swab samples is mostly colonized by the Vibrionaceae, family of Proteobacteria. The dolphin’s genital mucosa is colonized by several microorganisms equally distributed including Fusobacteriaceae and Leptotrichiaceae and Pasteurellaceae families. On the oral mucosa and tongue, once again is largely present the Pasteurellaceae family of bacteria, but also the Campylobacter family. The duodenal bulb is practically all colonized by the Peptostreptococcaceae bacteria, whereas the Colon fluid is constituted by the Pasteurellaceae family and Vibrionaceae family. By his turn, the blowhole sample is comprised mainly by Pasteurellaceae, while the skin lesion sample is colonized mostly by Cardiobacteriaceae and other marine microorganisms. With this work, sampling methodologies and sample processing conditions were established, which proved to be quite reproducible once replicates for each sample type were fairly consistent. |
|---|