Publicação
Syntrophic LCFA degradation: generating a high-energy carrier from low-energy metabolism
| Resumo: | For many years the interest in anaerobic degradation of lipids and long-chain fatty acids (LCFA) focused on technology and process developments. Attention to the microbiology of LCFA conversion was boosted by results showing that high methane yields could be obtained during anaerobic degradation of LCFA. The principle pathway of LCFA degradation is through -oxidation, producing acetate and hydrogen. For this conversion to be thermodynamically feasible, in methanogenic environments, acetogenic LCFA-degrading bacteria have to cooperate in syntrophy with hydrogen-consuming archaea. DGGE fingerprinting and 16S rRNA gene sequencing showed the importance of Syntrophomonas-like bacteria during batch and continuous degradation of unsaturated and saturated LCFA. The 7 species described thus far with the ability to grow on LCFA (with more than 12 carbon atoms), all belong to the families Syntrophomonadaceae and Syntrophaceae and, among these, only 4 species have the capability of utilizing mono- and/or polyunsaturated LCFA. Syntrophomonas zehnderi is able to degrade a wide range of saturated and unsaturated LCFA (C4 to C18) and could be detected in sludge samples from fed-batch and continuous reactors degrading oleate (monounsaturated C18). Batch bioaugmentation assays showed that addition of S. zenhderi could improve methane recovery from LCFA. The molecular mechanisms of anaerobic-LCFA degradation by S. zehnderi are being further studied by proteomics and whole genome sequencing. Identifying the components involved in the conversion of unsaturated LCFA-conversion is of particular interest. Related to this, the microbiology of oleate to palmitate (saturated C16) conversion by mixed cultures is also being studied using stable isotope probing and metaproteomics. |
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| Autores principais: | Sousa, D. Z. |
| Assunto: | LCFA syntrophy molecular ecology physiology |
| Ano: | 2011 |
| País: | Portugal |
| Tipo de documento: | outro |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| Resumo: | For many years the interest in anaerobic degradation of lipids and long-chain fatty acids (LCFA) focused on technology and process developments. Attention to the microbiology of LCFA conversion was boosted by results showing that high methane yields could be obtained during anaerobic degradation of LCFA. The principle pathway of LCFA degradation is through -oxidation, producing acetate and hydrogen. For this conversion to be thermodynamically feasible, in methanogenic environments, acetogenic LCFA-degrading bacteria have to cooperate in syntrophy with hydrogen-consuming archaea. DGGE fingerprinting and 16S rRNA gene sequencing showed the importance of Syntrophomonas-like bacteria during batch and continuous degradation of unsaturated and saturated LCFA. The 7 species described thus far with the ability to grow on LCFA (with more than 12 carbon atoms), all belong to the families Syntrophomonadaceae and Syntrophaceae and, among these, only 4 species have the capability of utilizing mono- and/or polyunsaturated LCFA. Syntrophomonas zehnderi is able to degrade a wide range of saturated and unsaturated LCFA (C4 to C18) and could be detected in sludge samples from fed-batch and continuous reactors degrading oleate (monounsaturated C18). Batch bioaugmentation assays showed that addition of S. zenhderi could improve methane recovery from LCFA. The molecular mechanisms of anaerobic-LCFA degradation by S. zehnderi are being further studied by proteomics and whole genome sequencing. Identifying the components involved in the conversion of unsaturated LCFA-conversion is of particular interest. Related to this, the microbiology of oleate to palmitate (saturated C16) conversion by mixed cultures is also being studied using stable isotope probing and metaproteomics. |
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