Detalhes bibliográficos
| Resumo: | Wickerhamomyces anomalus strain LBCM1105 was originally isolated from the wort of cachaça (the Brazilian fermented sugarcane juice-derived Brazilian spirit) and has been shown to grow exceptionally well at high amounts of glycerol. This paramount residue from the biodiesel industry is a promising cheap carbon source for yeast biotechnology. The assessment of the physiological traits underlying the W. anomalus glycerol consumption ability in opposition to Saccharomyces cerevisiae is presented. A new WaStl1 concentrative glycerol-H+ symporter with twice the affinity of S. cerevisiae was identified. As in this yeast, WaSTL1 is repressed by glucose and derepressed/induced by glycerol but much more highly expressed. Moreover, LBCM1105 aerobically growing on glycerol was found to produce ethanol, providing a redox escape to compensate the redox imbalance at the level of cyanide-resistant respiration (CRR) and glycerol 3P shuttle. This work is critical for understanding the utilization of glycerol by non-Saccharomyces yeasts being indispensable to consider their industrial application feeding on biodiesel residue. |
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| Autores principais: | da Cunha, Aureliano Claret |
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| Outros Autores: | Gomes, Lorena Soares; Godoy-Santos, Fernanda; Faria-Oliveira, Fábio; Teixeira, Janaína Aparecida; Sampaio, Geraldo Magela Santos; Trópia, Maria José Magalhães; Castro, Ieso Miranda; Lucas, Cândida; Brandão, Rogelio Lopes |
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| Assunto: | Aerobiosis Alcoholic Beverages Biofuels Biomass Bioreactors Brazil Candida Chromatography, High Pressure Liquid Cyanides Ethanol Fermentation Food Technology Glucose Glycerol Hydrogen-Ion Concentration Industrial Microbiology Kinetics Protons Saccharomyces cerevisiae Saccharomycetales Wickerhamomyces anomalus Glycerol metabolism Cachaca Biotechnological applications Glycerol transport STL1 |
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| Ano: | 2019 |
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| País: | Portugal |
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| Tipo de documento: | artigo |
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| Tipo de acesso: | acesso aberto |
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| Instituição associada: | Universidade do Minho |
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| Idioma: | inglês |
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| Origem: | RepositóriUM - Universidade do Minho |
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