Publicação
High-affinity transport, cyanide-resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalus
| Resumo: | Wickerhamomyces anomalus strain LBCM1105 was originally isolated from the wort of cachaça (the Brazilian fermented sugarcane juice-derived Brazilian spirit) and has been shown to grow exceptionally well at high amounts of glycerol. This paramount residue from the biodiesel industry is a promising cheap carbon source for yeast biotechnology. The assessment of the physiological traits underlying the W. anomalus glycerol consumption ability in opposition to Saccharomyces cerevisiae is presented. A new WaStl1 concentrative glycerol-H+ symporter with twice the affinity of S. cerevisiae was identified. As in this yeast, WaSTL1 is repressed by glucose and derepressed/induced by glycerol but much more highly expressed. Moreover, LBCM1105 aerobically growing on glycerol was found to produce ethanol, providing a redox escape to compensate the redox imbalance at the level of cyanide-resistant respiration (CRR) and glycerol 3P shuttle. This work is critical for understanding the utilization of glycerol by non-Saccharomyces yeasts being indispensable to consider their industrial application feeding on biodiesel residue. |
|---|---|
| Autores principais: | da Cunha, Aureliano Claret |
| Outros Autores: | Gomes, Lorena Soares; Godoy-Santos, Fernanda; Faria-Oliveira, Fábio; Teixeira, Janaína Aparecida; Sampaio, Geraldo Magela Santos; Trópia, Maria José Magalhães; Castro, Ieso Miranda; Lucas, Cândida; Brandão, Rogelio Lopes |
| Assunto: | Aerobiosis Alcoholic Beverages Biofuels Biomass Bioreactors Brazil Candida Chromatography, High Pressure Liquid Cyanides Ethanol Fermentation Food Technology Glucose Glycerol Hydrogen-Ion Concentration Industrial Microbiology Kinetics Protons Saccharomyces cerevisiae Saccharomycetales Wickerhamomyces anomalus Glycerol metabolism Cachaca Biotechnological applications Glycerol transport STL1 Ciências Naturais::Ciências Biológicas |
| Ano: | 2019 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867438840496521216 |
|---|---|
| author | da Cunha, Aureliano Claret |
| author2 | Gomes, Lorena Soares Godoy-Santos, Fernanda Faria-Oliveira, Fábio Teixeira, Janaína Aparecida Sampaio, Geraldo Magela Santos Trópia, Maria José Magalhães Castro, Ieso Miranda Lucas, Cândida Brandão, Rogelio Lopes |
| author2_role | author author author author author author author author author |
| author_facet | da Cunha, Aureliano Claret Gomes, Lorena Soares Godoy-Santos, Fernanda Faria-Oliveira, Fábio Teixeira, Janaína Aparecida Sampaio, Geraldo Magela Santos Trópia, Maria José Magalhães Castro, Ieso Miranda Lucas, Cândida Brandão, Rogelio Lopes |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"da Cunha, Aureliano Claret\"},{\"Person.name\":\"Gomes, Lorena Soares\"},{\"Person.name\":\"Godoy-Santos, Fernanda\"},{\"Person.name\":\"Faria-Oliveira, Fábio\"},{\"Person.name\":\"Teixeira, Janaína Aparecida\"},{\"Person.name\":\"Sampaio, Geraldo Magela Santos\"},{\"Person.name\":\"Trópia, Maria José Magalhães\"},{\"Person.name\":\"Castro, Ieso Miranda\"},{\"Person.name\":\"Lucas, Cândida\"},{\"Person.name\":\"Brandão, Rogelio Lopes\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | da Cunha, Aureliano Claret Gomes, Lorena Soares Godoy-Santos, Fernanda Faria-Oliveira, Fábio Teixeira, Janaína Aparecida Sampaio, Geraldo Magela Santos Trópia, Maria José Magalhães Castro, Ieso Miranda Lucas, Cândida Brandão, Rogelio Lopes |
| datacite.date.Accepted.fl_str_mv | 2019-05-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2021-06-16T17:02:50Z |
| datacite.date.embargoed.fl_str_mv | 2021-06-16T17:02:50Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Aerobiosis Alcoholic Beverages Biofuels Biomass Bioreactors Brazil Candida Chromatography, High Pressure Liquid Cyanides Ethanol Fermentation Food Technology Glucose Glycerol Hydrogen-Ion Concentration Industrial Microbiology Kinetics Protons Saccharomyces cerevisiae Saccharomycetales Wickerhamomyces anomalus Glycerol metabolism Cachaca Biotechnological applications Glycerol transport STL1 Ciências Naturais::Ciências Biológicas |
| datacite.titles.title.fl_str_mv | High-affinity transport, cyanide-resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalus |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | da Cunha, Aureliano Claret Gomes, Lorena Soares Godoy-Santos, Fernanda Faria-Oliveira, Fábio Teixeira, Janaína Aparecida Sampaio, Geraldo Magela Santos Trópia, Maria José Magalhães Castro, Ieso Miranda Lucas, Cândida Brandão, Rogelio Lopes |
| dc.date.Accepted.fl_str_mv | 2019-05-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2021-06-16T17:02:50Z |
| dc.date.embargoed.fl_str_mv | 2021-06-16T17:02:50Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/73415 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Springer |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Aerobiosis Alcoholic Beverages Biofuels Biomass Bioreactors Brazil Candida Chromatography, High Pressure Liquid Cyanides Ethanol Fermentation Food Technology Glucose Glycerol Hydrogen-Ion Concentration Industrial Microbiology Kinetics Protons Saccharomyces cerevisiae Saccharomycetales Wickerhamomyces anomalus Glycerol metabolism Cachaca Biotechnological applications Glycerol transport STL1 Ciências Naturais::Ciências Biológicas |
| dc.title.fl_str_mv | High-affinity transport, cyanide-resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalus |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Wickerhamomyces anomalus strain LBCM1105 was originally isolated from the wort of cachaça (the Brazilian fermented sugarcane juice-derived Brazilian spirit) and has been shown to grow exceptionally well at high amounts of glycerol. This paramount residue from the biodiesel industry is a promising cheap carbon source for yeast biotechnology. The assessment of the physiological traits underlying the W. anomalus glycerol consumption ability in opposition to Saccharomyces cerevisiae is presented. A new WaStl1 concentrative glycerol-H+ symporter with twice the affinity of S. cerevisiae was identified. As in this yeast, WaSTL1 is repressed by glucose and derepressed/induced by glycerol but much more highly expressed. Moreover, LBCM1105 aerobically growing on glycerol was found to produce ethanol, providing a redox escape to compensate the redox imbalance at the level of cyanide-resistant respiration (CRR) and glycerol 3P shuttle. This work is critical for understanding the utilization of glycerol by non-Saccharomyces yeasts being indispensable to consider their industrial application feeding on biodiesel residue. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/7b24338f-eee0-4735-a1a8-ad00f36f47ae/download |
| id | rum_fbaeadfcefe749cc3ccc3d201bbe1eda |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/73415 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/73415 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | da Cunha, Aureliano Claret Gomes, Lorena Soares Godoy-Santos, Fernanda Faria-Oliveira, Fábio Teixeira, Janaína Aparecida Sampaio, Geraldo Magela Santos Trópia, Maria José Magalhães Castro, Ieso Miranda Lucas, Cândida Brandão, Rogelio Lopes |
| publishDate | 2019 |
| publisher.none.fl_str_mv | Springer |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engSpringerporWickerhamomyces anomalus strain LBCM1105 was originally isolated from the wort of cachaça (the Brazilian fermented sugarcane juice-derived Brazilian spirit) and has been shown to grow exceptionally well at high amounts of glycerol. This paramount residue from the biodiesel industry is a promising cheap carbon source for yeast biotechnology. The assessment of the physiological traits underlying the W. anomalus glycerol consumption ability in opposition to Saccharomyces cerevisiae is presented. A new WaStl1 concentrative glycerol-H+ symporter with twice the affinity of S. cerevisiae was identified. As in this yeast, WaSTL1 is repressed by glucose and derepressed/induced by glycerol but much more highly expressed. Moreover, LBCM1105 aerobically growing on glycerol was found to produce ethanol, providing a redox escape to compensate the redox imbalance at the level of cyanide-resistant respiration (CRR) and glycerol 3P shuttle. This work is critical for understanding the utilization of glycerol by non-Saccharomyces yeasts being indispensable to consider their industrial application feeding on biodiesel residue.application/pdfporHigh-affinity transport, cyanide-resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalusda Cunha, Aureliano ClaretGomes, Lorena SoaresGodoy-Santos, FernandaFaria-Oliveira, FábioTeixeira, Janaína AparecidaSampaio, Geraldo Magela SantosTrópia, Maria José MagalhãesCastro, Ieso MirandaLucas, CândidaBrandão, Rogelio LopesHostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATIONAureliano Claret da Cunha, Lorena Soares Gomes, Fernanda Godoy-Santos, Fábio Faria-Oliveira, Janaína Aparecida Teixeira, Geraldo Magela Santos Sampaio, Maria José Magalhães Trópia, Ieso Miranda Castro, Cândida Lucas, Rogelio Lopes Brandão, High-affinity transport, cyanide-resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalus, Journal of Industrial Microbiology and Biotechnology, Volume 46, Issue 5, 1 May 2019, Pages 709–723, https://doi.org/10.1007/s10295-018-02119-5PMID30680472ISSNIsPartOf1367-5435DOIIsPartOf10.1007/s10295-018-02119-52021-06-16T17:02:50Z2019-052019-05-01T00:00:00ZHandlehttps://hdl.handle.net/1822/73415http://purl.org/coar/access_right/c_abf2open accessAerobiosisAlcoholic BeveragesBiofuelsBiomassBioreactorsBrazilCandidaChromatography, High Pressure LiquidCyanidesEthanolFermentationFood TechnologyGlucoseGlycerolHydrogen-Ion ConcentrationIndustrial MicrobiologyKineticsProtonsSaccharomyces cerevisiaeSaccharomycetalesWickerhamomyces anomalusGlycerol metabolismCachacaBiotechnological applicationsGlycerol transportSTL1http://www.oecd.org/science/inno/38235147.pdfFields of Science and Technology (FOS)Ciências Naturais::Ciências Biológicas2845165 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/7b24338f-eee0-4735-a1a8-ad00f36f47ae/download |
| spellingShingle | High-affinity transport, cyanide-resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalus da Cunha, Aureliano Claret Aerobiosis Alcoholic Beverages Biofuels Biomass Bioreactors Brazil Candida Chromatography, High Pressure Liquid Cyanides Ethanol Fermentation Food Technology Glucose Glycerol Hydrogen-Ion Concentration Industrial Microbiology Kinetics Protons Saccharomyces cerevisiae Saccharomycetales Wickerhamomyces anomalus Glycerol metabolism Cachaca Biotechnological applications Glycerol transport STL1 Ciências Naturais::Ciências Biológicas |
| status | SINGLETON |
| subject.fl_str_mv | Aerobiosis Alcoholic Beverages Biofuels Biomass Bioreactors Brazil Candida Chromatography, High Pressure Liquid Cyanides Ethanol Fermentation Food Technology Glucose Glycerol Hydrogen-Ion Concentration Industrial Microbiology Kinetics Protons Saccharomyces cerevisiae Saccharomycetales Wickerhamomyces anomalus Glycerol metabolism Cachaca Biotechnological applications Glycerol transport STL1 |
| subject.other.fl_str_mv | Ciências Naturais::Ciências Biológicas |
| title | High-affinity transport, cyanide-resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalus |
| title_full | High-affinity transport, cyanide-resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalus |
| title_fullStr | High-affinity transport, cyanide-resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalus |
| title_full_unstemmed | High-affinity transport, cyanide-resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalus |
| title_short | High-affinity transport, cyanide-resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalus |
| title_sort | High-affinity transport, cyanide-resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalus |
| topic | Aerobiosis Alcoholic Beverages Biofuels Biomass Bioreactors Brazil Candida Chromatography, High Pressure Liquid Cyanides Ethanol Fermentation Food Technology Glucose Glycerol Hydrogen-Ion Concentration Industrial Microbiology Kinetics Protons Saccharomyces cerevisiae Saccharomycetales Wickerhamomyces anomalus Glycerol metabolism Cachaca Biotechnological applications Glycerol transport STL1 Ciências Naturais::Ciências Biológicas |
| topic_facet | Aerobiosis Alcoholic Beverages Biofuels Biomass Bioreactors Brazil Candida Chromatography, High Pressure Liquid Cyanides Ethanol Fermentation Food Technology Glucose Glycerol Hydrogen-Ion Concentration Industrial Microbiology Kinetics Protons Saccharomyces cerevisiae Saccharomycetales Wickerhamomyces anomalus Glycerol metabolism Cachaca Biotechnological applications Glycerol transport STL1 Ciências Naturais::Ciências Biológicas |
| url | https://hdl.handle.net/1822/73415 |
| visible | 1 |