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Novel Insights into the Catalytic Mechanism of the W-FdhAB from Desulfovibrio vulgaris Hildenborough

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Detalhes bibliográficos
Resumo:"It is known that the world is facing the negative environmental impacts of fossil fuel utilization. In 2023, a total of 37.4 Gt of CO2 was released to the atmosphere, having erects in atmospheric warming, precipitation patterns, and sea level rise. The use of CO2 as a feedstock to generate added-value products is an alternative to decrease atmospheric CO2 levels in order to create a CO2 circular economy. Formate is an example of one of those products, being considered an alternative liquid fuel, which is safer to store and transport than H2. The W-containing formate dehydrogenase AB (FdhAB) from Desulfovibrio vulgaris Hildenborough catalyzes the interconversion of CO2 and formate. This enzyme has interesting characteristics amongst Fdhs – it has a high CO2 reduction activity, a simple structure, it can be handled in aerobic conditions and has an established expression system.(...)"
Autores principais:Rebelo Manuel, Rita
Assunto:CO2 circular economy formate W-FdhAB catalytic mechanism WFdhAB O2-inactivation W-FdhAB variants
Ano:2024
País:Portugal
Tipo de documento:tese de doutoramento
Tipo de acesso:acesso embargado
Instituição associada:Universidade Nova de Lisboa
Idioma:inglês
Origem:Repositório Institucional da UNL
Descrição
Resumo:"It is known that the world is facing the negative environmental impacts of fossil fuel utilization. In 2023, a total of 37.4 Gt of CO2 was released to the atmosphere, having erects in atmospheric warming, precipitation patterns, and sea level rise. The use of CO2 as a feedstock to generate added-value products is an alternative to decrease atmospheric CO2 levels in order to create a CO2 circular economy. Formate is an example of one of those products, being considered an alternative liquid fuel, which is safer to store and transport than H2. The W-containing formate dehydrogenase AB (FdhAB) from Desulfovibrio vulgaris Hildenborough catalyzes the interconversion of CO2 and formate. This enzyme has interesting characteristics amongst Fdhs – it has a high CO2 reduction activity, a simple structure, it can be handled in aerobic conditions and has an established expression system.(...)"