Publicação
Development of mesoporous bioglass-based systems for controlled delivery of cytotoxic drugs
| Resumo: | Cancer remains a leading global cause of death, with over 10 million deaths annually. Osteosarcoma, a primary bone cancer, predominantly affects children and young adults, and it typically requires conventional therapies, such as radiotherapy, chemotherapy, and surgery. However, these treatments cause significant side effects and carry the risk of cancer recurrence. This study presents the synthesis and analysis of mesoporous bioactive glasses (MBGs) doped with varying copper concentrations - 0 % (BG0), 2 % (BG2), and 5 % (BG5) mol.%, using the sol-gel method, assisted by the Evaporation-Induced Self-Assembly (EISA) technique. 25 mg of MBGs were loaded with 0.1, 0.2, and 0.4 mg/mL of doxorubicin (DOX) for controlled drug delivery studies. Characterization of the MBGs revealed median particle sizes below 3 μm, with specific surface areas ranging from 187 to 367 m²/g, pore volumes between 0.227 and 0.393 cm³/g, and pore diameters between 3.97 and 4.81 nm. Bioactivity studies demonstrated the formation of a hydroxycarbonate apatite layer within 7 days, highlighting its potential to promote bone regeneration. DOX encapsulation efficiencies ranged from 75.6 % for BG0 with 0.1 mg/mL of DOX to 97.1 % for BG2 with 0.4 mg/mL of DOX. The highest cumulative drug release was 18.3 % after 7 days, being inversely proportional to the copper concentration within the samples. The addition of copper enhanced anti-bacterial properties against S. aureus andE. coli bacteria, though it also increased the cytotoxicity of the samples. This study demonstrates the potential of multifunctional MBGs for bone regeneration, controlled drug delivery, and anti-bacterial activity, providing promising results as a comple- mentary therapy for the treatment of bone cancer. |
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| Autores principais: | Cordas, Alexandra Ferreira |
| Assunto: | Bone regeneration copper doxorubicin drug delivery mesoporous bioactive glass. |
| Ano: | 2024 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| Resumo: | Cancer remains a leading global cause of death, with over 10 million deaths annually. Osteosarcoma, a primary bone cancer, predominantly affects children and young adults, and it typically requires conventional therapies, such as radiotherapy, chemotherapy, and surgery. However, these treatments cause significant side effects and carry the risk of cancer recurrence. This study presents the synthesis and analysis of mesoporous bioactive glasses (MBGs) doped with varying copper concentrations - 0 % (BG0), 2 % (BG2), and 5 % (BG5) mol.%, using the sol-gel method, assisted by the Evaporation-Induced Self-Assembly (EISA) technique. 25 mg of MBGs were loaded with 0.1, 0.2, and 0.4 mg/mL of doxorubicin (DOX) for controlled drug delivery studies. Characterization of the MBGs revealed median particle sizes below 3 μm, with specific surface areas ranging from 187 to 367 m²/g, pore volumes between 0.227 and 0.393 cm³/g, and pore diameters between 3.97 and 4.81 nm. Bioactivity studies demonstrated the formation of a hydroxycarbonate apatite layer within 7 days, highlighting its potential to promote bone regeneration. DOX encapsulation efficiencies ranged from 75.6 % for BG0 with 0.1 mg/mL of DOX to 97.1 % for BG2 with 0.4 mg/mL of DOX. The highest cumulative drug release was 18.3 % after 7 days, being inversely proportional to the copper concentration within the samples. The addition of copper enhanced anti-bacterial properties against S. aureus andE. coli bacteria, though it also increased the cytotoxicity of the samples. This study demonstrates the potential of multifunctional MBGs for bone regeneration, controlled drug delivery, and anti-bacterial activity, providing promising results as a comple- mentary therapy for the treatment of bone cancer. |
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