Detalhes bibliográficos
| Resumo: | Adenosine-5-triphosphate (ATP) is the main energy vector in biological systems, thus its regeneration is an important issue for the application of many enzymes of interest in biocatalysis and synthetic biology. We have developed an electroenzymatic ATP regeneration system consisting in a gold electrode modified with a floating phospholipid bilayer that allows coupling the catalytic activity of two membrane-bound enzymes: NiFeSe hydrogenase from Desulfovibrio vulgaris and F1Fo-ATP synthase from Escherichia coli. Thus, H2 is used as a fuel for producing ATP. This electro-enzymatic assembly is studied as ATP regeneration system of phosphorylation reactions catalysed by kinases, such as hexokinase and NAD+-kinase for respectively producing glucose-6-phosphate and NADP+. |
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| Autores principais: | García-Molina, Gabriel |
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| Outros Autores: | Natale, Paolo; Coito, Ana M.; Cava, Daniel G.; A. C. Pereira, Inés; López-Montero, Iván; Vélez, Marisela; Pita, Marcos; De Lacey, Antonio L. |
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| Assunto: | ATP regeneration ATP-synthase Hydrogenase Kinase Phosphorylation Biophysics Physical and Theoretical Chemistry Electrochemistry |
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| Ano: | 2023 |
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| País: | Portugal |
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| Tipo de documento: | artigo |
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| Tipo de acesso: | acesso aberto |
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| Instituição associada: | Universidade Nova de Lisboa |
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| Idioma: | inglês |
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| Origem: | Repositório Institucional da UNL |
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