Publicação
Detection of drug resistant mycobacterium tuberculosis strains using kit SIRE nitratase®
| Resumo: | (1) Background: The Commercial Kit SIRE Nitratase® PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2) Methods: To determine its accuracy, the proportion methods in Lowenstein Jensen medium or the BACTECTMMGITTM960 system was used as a gold standard. (3) Results: The study revealed that the respective accuracies of the kit with 190 M. tuberculosis clinical isolates, using the proportion methods in Lowenstein Jensen medium or BACTECTMMGITTM960 system as a gold standard, were 93.9% and 94.6%, 96.9% and 94.6%, 98.0% and 97.8%, and 98.0% and 98.9%, for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. (4) Conclusion: Thus, the kit can rapidly screen resistance to streptomycin, isoniazid, rifampicin, and ethambutol. Additionally, it does not require sophisticated equipment; hence, it can be easily used in the laboratories of low and middle income countries. |
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| Autores principais: | de Miranda, Silvana Spindola |
| Outros Autores: | de Almeida, Isabela Neves; de Fátima Filardi Oliveira Mansur, Maria; de Assis Figueredo, Lida Jouca; da Silva Carvalho, Wânia; Hadaad, João Paulo Amaral; de Lourdes do Carmo Guimarães Diniz, Jaciara; von Groll, Andrea; da Silva, Pedro Almeida; Lopes, Maria Luiza; dos Santos, Marcelo Cordeiro; Brito, Alexandra; de Queiroz Mello, Fernanda Carvalho; da Silva Santos Malaquias, Thiago; Croda, Julio; Pinhata, Juliana Maira Watanabe; de Oliveira, Rosângela Siqueira; Chimara, Erica; Rossetti, Maria Lúcia; Halon, Maria Laura; Lourenço, Maria Cristina; de Melo Medeiros, Reginalda Ferreira; Montes, Fátima Cristina Onofre Fandinho; Machado, Diana; Viveiros, Miguel; Kritski, Afrânio Lineu |
| Assunto: | MDR Nitrate reductase Technology Tuberculosis Biochemistry, Genetics and Molecular Biology (miscellaneous) Infectious Diseases Microbiology SDG 3 - Good Health and Well-being |
| Ano: | 2020 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| Resumo: | (1) Background: The Commercial Kit SIRE Nitratase® PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2) Methods: To determine its accuracy, the proportion methods in Lowenstein Jensen medium or the BACTECTMMGITTM960 system was used as a gold standard. (3) Results: The study revealed that the respective accuracies of the kit with 190 M. tuberculosis clinical isolates, using the proportion methods in Lowenstein Jensen medium or BACTECTMMGITTM960 system as a gold standard, were 93.9% and 94.6%, 96.9% and 94.6%, 98.0% and 97.8%, and 98.0% and 98.9%, for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. (4) Conclusion: Thus, the kit can rapidly screen resistance to streptomycin, isoniazid, rifampicin, and ethambutol. Additionally, it does not require sophisticated equipment; hence, it can be easily used in the laboratories of low and middle income countries. |
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