Publicação
Fast centrifugal partition chromatography for C-Phycocyanin purification from Limnospira platensis using eutectic solvent-based biphasic systems
| Resumo: | The development of sustainable separation strategies for high-value bioproducts from microalgae is essential for advancing integrated biorefinery approaches. This study investigates the use of eutectic solvent (ES)-based biphasic systems for the selective separation of C-phycocyanin (C-PC), a water-soluble pigment-protein complex, from the crude extract of Limnospira platensis (L. platensis). Two ESs combinations were selected based on phase stability and partitioning behavior: (i) a hydrophilic ES composed of glucose:glycerol:H2O (1:2:2.5) diluted with deionized water and paired with the hydrophobic ES decanoic acid:menthol (2:1), and (ii) the same hydrophilic ES diluted with phosphate buffer solution (14 wt%) and combined with a hydrophobic ES comprising octanoic acid:decanoic acid:dodecanoic acid (3:2:1). These systems were evaluated using ultra-Fast Centrifugal Partition Chromatography (FCPC) under optimized conditions to enhance phase retention and resolution. The first system demonstrated effective separation with partition coefficients of 1.53 for C-PC and 0.50 for chlorophyll, achieving high purity at low pigment concentrations (15.9 mgC-PC⋅L−1 purity 5 times higher towards total proteins and chlorophylls). In contrast, the second system yielded more concentrated fractions (up to 615.9 mgC-PC⋅L−1 purity 5 and 0.87 times higher towards total proteins and chlorophylls, respectively) but exhibited low selectivity, with both pigments presenting similar partition behavior (K ≈ 0.03). Overall, the results feature the potential of ES-based biphasic systems for pigment purification and highlight the trade-offs between resolution, purity, and concentration. |
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| Autores principais: | Pereira, Bruno A. |
| Outros Autores: | Conde, Alexandra; Valente, João Vasco; Freire, Mara G.; Ventura, Sónia P. M. |
| Assunto: | C-phycocyanin ES-based biphasic systems Fast centrifugal partition chromatography Pigment separation Analytical Chemistry Filtration and Separation |
| Ano: | 2026 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| Resumo: | The development of sustainable separation strategies for high-value bioproducts from microalgae is essential for advancing integrated biorefinery approaches. This study investigates the use of eutectic solvent (ES)-based biphasic systems for the selective separation of C-phycocyanin (C-PC), a water-soluble pigment-protein complex, from the crude extract of Limnospira platensis (L. platensis). Two ESs combinations were selected based on phase stability and partitioning behavior: (i) a hydrophilic ES composed of glucose:glycerol:H2O (1:2:2.5) diluted with deionized water and paired with the hydrophobic ES decanoic acid:menthol (2:1), and (ii) the same hydrophilic ES diluted with phosphate buffer solution (14 wt%) and combined with a hydrophobic ES comprising octanoic acid:decanoic acid:dodecanoic acid (3:2:1). These systems were evaluated using ultra-Fast Centrifugal Partition Chromatography (FCPC) under optimized conditions to enhance phase retention and resolution. The first system demonstrated effective separation with partition coefficients of 1.53 for C-PC and 0.50 for chlorophyll, achieving high purity at low pigment concentrations (15.9 mgC-PC⋅L−1 purity 5 times higher towards total proteins and chlorophylls). In contrast, the second system yielded more concentrated fractions (up to 615.9 mgC-PC⋅L−1 purity 5 and 0.87 times higher towards total proteins and chlorophylls, respectively) but exhibited low selectivity, with both pigments presenting similar partition behavior (K ≈ 0.03). Overall, the results feature the potential of ES-based biphasic systems for pigment purification and highlight the trade-offs between resolution, purity, and concentration. |
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