Publicação
Transformation of grey poplar to introduce resistance to Phytophthora sp.
| Resumo: | "Phytophthora cinnamomi is a widespread, aggressive and destructive plant pathogen with a wide variety of economically-relevant host species. One such species is the chestnut tree, Castanea sativa, which has provided an important European food and wood source since the medieval times. A previous transcriptomics and genomics study identified differentially expressed genes on resistant and susceptible chestnut varieties. One of the most promising genes was the GINKBILOBIN2-like (GNK2-like), whose protein, secreted by the roots of Castanea crenata, has a known antifungal domain. This work aimed to transform grey poplar (Populus tremula x alba), a model for trees, with an overexpressed Cast_GNK2-like gene in order to validate the anti-Phytophthora activity of this protein through a heterologous system.(...)" |
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| Autores principais: | Marques, Rita M |
| Assunto: | Validação funcional Populus tremula x alba Phytophthora cinnamomi Phytophthora cactorum |
| Ano: | 2020 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| Resumo: | "Phytophthora cinnamomi is a widespread, aggressive and destructive plant pathogen with a wide variety of economically-relevant host species. One such species is the chestnut tree, Castanea sativa, which has provided an important European food and wood source since the medieval times. A previous transcriptomics and genomics study identified differentially expressed genes on resistant and susceptible chestnut varieties. One of the most promising genes was the GINKBILOBIN2-like (GNK2-like), whose protein, secreted by the roots of Castanea crenata, has a known antifungal domain. This work aimed to transform grey poplar (Populus tremula x alba), a model for trees, with an overexpressed Cast_GNK2-like gene in order to validate the anti-Phytophthora activity of this protein through a heterologous system.(...)" |
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