Publicação
SARS-CoV-2 Infection in Children
| Resumo: | Children with COVID-19 typically experience milder symptoms and lower hospitalization rates, though severe cases do occur. Understanding age-related immune responses is crucial for future preparedness. We characterized immune response dynamics to SARS-CoV-2 in 145 samples from 119 pediatric patients (<18 years) with confirmed infection, assessed at four distinct time points: <14 days, 14 days-3 months, 3-6 months, and 6-12 months post-infection. At infection, patients presented increased activated T-cells, higher levels of exhaustion (i.e., PD-1 +), lower numbers of unswitched memory B-cells, and increased antibody-secreting cells (ASCs). Both humoral and cellular anti-SARS-CoV-2 responses increased over time (all patients showed measurable responses in the last assessment). Asymptomatic/mildly symptomatic patients (58.6%) showed increased specific cellular responses from infection onwards, along with enriched memory B-cell subsets (but not ASCs), and distinct T-cell activation profiles. Children with severe disease were younger, predominantly boys, displayed altered T/B-cell ratios, and reduced PHA responses when infected. Compared to adolescents, younger children showed lower antibody titers and weaker cellular responses to SARS-CoV-2, possibly underlining the higher prevalence of severe manifestations in younger children. Our study illustrates important age-, gender-, and disease severity-dependent variations in immune responses to SARS-CoV-2, which can be helpful in improving patient management and immunization strategies adjusted to age groups. |
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| Autores principais: | Martins, Catarina Gregório |
| Outros Autores: | Ângelo-Dias, Miguel; Martins, Catarina; Chasqueira, Maria de Jesus; Brito, Maria João; Ângelo-Dias, Miguel; Silva, Tiago Milheiro; Matos, Maria Vitória; Chasqueira, Maria-Jesus; Lopes, Maria Teresa; Crespo, Hélio; Mata, Mariana; Borrego, Luís Miguel; Paixão, Paulo; Lopes, Maria Teresa; BORREGO, LUIS MIGUEL; Paixão, Paulo |
| Assunto: | Humans COVID-19/immunology Child Male Female SARS-CoV-2/immunology Adolescent Child, Preschool Antibodies, Viral/blood T-Lymphocytes/immunology Infant Host-Pathogen Interactions/immunology B-Lymphocytes/immunology Immunity, Cellular Memory B Cells/immunology Lymphocyte Activation |
| Ano: | 2025 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| Resumo: | Children with COVID-19 typically experience milder symptoms and lower hospitalization rates, though severe cases do occur. Understanding age-related immune responses is crucial for future preparedness. We characterized immune response dynamics to SARS-CoV-2 in 145 samples from 119 pediatric patients (<18 years) with confirmed infection, assessed at four distinct time points: <14 days, 14 days-3 months, 3-6 months, and 6-12 months post-infection. At infection, patients presented increased activated T-cells, higher levels of exhaustion (i.e., PD-1 +), lower numbers of unswitched memory B-cells, and increased antibody-secreting cells (ASCs). Both humoral and cellular anti-SARS-CoV-2 responses increased over time (all patients showed measurable responses in the last assessment). Asymptomatic/mildly symptomatic patients (58.6%) showed increased specific cellular responses from infection onwards, along with enriched memory B-cell subsets (but not ASCs), and distinct T-cell activation profiles. Children with severe disease were younger, predominantly boys, displayed altered T/B-cell ratios, and reduced PHA responses when infected. Compared to adolescents, younger children showed lower antibody titers and weaker cellular responses to SARS-CoV-2, possibly underlining the higher prevalence of severe manifestations in younger children. Our study illustrates important age-, gender-, and disease severity-dependent variations in immune responses to SARS-CoV-2, which can be helpful in improving patient management and immunization strategies adjusted to age groups. |
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