Publicação
Substrate-binding proteins as amino acid sensors inside Cytolysin A
| Resumo: | Amino acids are important biomarkers within the context of several human diseases. However, continuous measurement of their levels is hard due to the high-cost complex machinery involved. Biological nanopores are one of the emerging biosensing alternatives suitable for this purpose. In this thesis, ClyA-AS type I was used along with engineered protein sensors for glutamine, asparagine, leucine, methionine and cysteine. We show that discrimination and quantification of glutamine, leucine and cysteine is possible, and further provide favorable insights for the future use of this biosensing approach with different engineered protein sensors. |
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| Autores principais: | Teixeira, Pedro Gustavo Ferreira Curto Pedroso |
| Assunto: | Amino acids Biosensing Nanopores Substrate-binding proteins Dissociation Constant |
| Ano: | 2025 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso embargado |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| Resumo: | Amino acids are important biomarkers within the context of several human diseases. However, continuous measurement of their levels is hard due to the high-cost complex machinery involved. Biological nanopores are one of the emerging biosensing alternatives suitable for this purpose. In this thesis, ClyA-AS type I was used along with engineered protein sensors for glutamine, asparagine, leucine, methionine and cysteine. We show that discrimination and quantification of glutamine, leucine and cysteine is possible, and further provide favorable insights for the future use of this biosensing approach with different engineered protein sensors. |
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