Publicação
Glass Microfluidic platforms for molecular detection by SERS
| Resumo: | Nowadays due to increasing concerns in public health, technologies have been developed for the sensitive detection of food contaminants. Since current methods are time-consum-ing, expensive and complex to operate, this project has as the objective to develop a mi-crofluidics device for surface enhanced Raman spectroscopy (SERS). SERS consists in a simple, robust, sensitive and fast technique for detection of ves-tigial components that can be advantageously adapted to the detection of food contami-nants. In order to develop an appropriate substrate for SERS, a study was performed of different parameters for the hydrothermal synthesis of zinc oxide nanorods (temperature, time and pH). Afterwards, they were covered with silver nanoparticles with different thicknesses (2, 4, 6 e 8 nm) deposited by physical vapour deposition (PVD), originating the substrate Ag NPs@ZnONRs. The efficiency of the different substrates was similar, regardless the thickness of silver, with enhancement factor (EF) values around 105~106. Simultaneously, obelisk nanorods were developed with good SERS efficiency, and EFs around 106. Finally, it was created a microfluidics device in PDMS, which allowed to observe the SERS signal of a rhodamine-6G solution in its interior. The assays performed in the work allowed to infer the behaviour of SERS signal with different geometric parameters of components of SERS substrates. |
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| Autores principais: | Ferreira, Bernardo Matos Alves Costa |
| Assunto: | Microfluidics Surface enhanced Raman spectroscopy (SERS) Zinc oxide (ZnO) Nanorods |
| Ano: | 2016 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| Resumo: | Nowadays due to increasing concerns in public health, technologies have been developed for the sensitive detection of food contaminants. Since current methods are time-consum-ing, expensive and complex to operate, this project has as the objective to develop a mi-crofluidics device for surface enhanced Raman spectroscopy (SERS). SERS consists in a simple, robust, sensitive and fast technique for detection of ves-tigial components that can be advantageously adapted to the detection of food contami-nants. In order to develop an appropriate substrate for SERS, a study was performed of different parameters for the hydrothermal synthesis of zinc oxide nanorods (temperature, time and pH). Afterwards, they were covered with silver nanoparticles with different thicknesses (2, 4, 6 e 8 nm) deposited by physical vapour deposition (PVD), originating the substrate Ag NPs@ZnONRs. The efficiency of the different substrates was similar, regardless the thickness of silver, with enhancement factor (EF) values around 105~106. Simultaneously, obelisk nanorods were developed with good SERS efficiency, and EFs around 106. Finally, it was created a microfluidics device in PDMS, which allowed to observe the SERS signal of a rhodamine-6G solution in its interior. The assays performed in the work allowed to infer the behaviour of SERS signal with different geometric parameters of components of SERS substrates. |
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