Detalhes bibliográficos
| Resumo: | The high global burden of over one million annual lethal fungal infections reflects a lack of protective vaccines, late diagnosis and inadequate chemotherapy. Here, we have generated a unique set of fully human anti-Candida monoclonal antibodies (mAbs) with diagnostic and therapeutic potential by expressing recombinant antibodies from genes cloned from the B cells of patients suffering from candidiasis. Single class switched memory B cells isolated from donors serum-positive for anti-Candida IgG were differentiated in vitro and screened against recombinant Candida albicans Hyr1 cell wall protein and whole fungal cell wall preparations. Antibody genes from Candida-reactive B cell cultures were cloned and expressed in Expi293F human embryonic kidney cells to generate a panel of human recombinant anti-Candida mAbs that demonstrate morphology-specific, high avidity binding to the cell wall. The species-specific and pan-Candida mAbs generated through this technology display favourable properties for diagnostics, strong opsono-phagocytic activity of macrophages in vitro, and protection in a murine model of disseminated candidiasis. |
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| Autores principais: | Rudkin, Fiona M. |
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| Outros Autores: | Raziunaite, Ingrida; Workman, Hillary; Essono, Sosthene; Belmonte, Rodrigo; MacCallum, Donna M.; Johnson, Elizabeth M.; Silva, Lisete M.; Palma, Angelina S.; Feizi, Ten; Jensen, Allan; Erwig, Lars P.; Gow, Neil A.R. |
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| Assunto: | General Chemistry General Biochemistry,Genetics and Molecular Biology General Physics and Astronomy SDG 3 - Good Health and Well-being |
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| Ano: | 2018 |
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| País: | Portugal |
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| Tipo de documento: | artigo |
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| Tipo de acesso: | acesso aberto |
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| Instituição associada: | Universidade Nova de Lisboa |
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| Idioma: | inglês |
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| Origem: | Repositório Institucional da UNL |
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