Publicação
Stereological Estimation of Mean Nuclear Volume as a Prognostic Factor in Canine Mast Cell Tumors
| Resumo: | Cutaneous mast cell tumour (MCT) Patnaik and Kiupel grading schemes rely on qualitative and semiquantitative features susceptible to interobserver variability. Stereological estimation of volume-weighted mean nuclear volume (MNV) provides information about both size and variability of nuclear size, which has been proven to have a prognostic value in other solid tumours. The objective was to compare MNV with MCT grade and biological behaviour. Fifty-six MCTs were graded according to Patnaik and Kiupel by consensus of three experienced pathologists.Clinical history of dogs treated with surgical excision alone was collected with a minimum follow-up period of 1 year (n=31).MNV was estimated using the point-intercept method on vertical sections in 10 microscopical fields, with an approximately constant distance proportional to overall sectional area. Animals were divided according to outcome: group 1, no recurrence; group 2, local recurrence, lymph node or distant metastasis. The present study suggests that estimation of MNV on routine histological sections may objectively improve the detection of more aggressive MCTs. |
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| Autores principais: | Casanova, M |
| Outros Autores: | Branco, S; Veiga, I; Faísca, P |
| Assunto: | mast cell tumours canine mean nuclear volume |
| Ano: | 2021 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Évora |
| Idioma: | português |
| Origem: | Repositório Científico da Universidade de Évora |
| Resumo: | Cutaneous mast cell tumour (MCT) Patnaik and Kiupel grading schemes rely on qualitative and semiquantitative features susceptible to interobserver variability. Stereological estimation of volume-weighted mean nuclear volume (MNV) provides information about both size and variability of nuclear size, which has been proven to have a prognostic value in other solid tumours. The objective was to compare MNV with MCT grade and biological behaviour. Fifty-six MCTs were graded according to Patnaik and Kiupel by consensus of three experienced pathologists.Clinical history of dogs treated with surgical excision alone was collected with a minimum follow-up period of 1 year (n=31).MNV was estimated using the point-intercept method on vertical sections in 10 microscopical fields, with an approximately constant distance proportional to overall sectional area. Animals were divided according to outcome: group 1, no recurrence; group 2, local recurrence, lymph node or distant metastasis. The present study suggests that estimation of MNV on routine histological sections may objectively improve the detection of more aggressive MCTs. |
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