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Quercus suber MYB1 transcription factor - an in vivo approach for the elucidation of its regulatory networks

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Detalhes bibliográficos
Resumo:The study of transcription factors (TFs) can reveal important players behind a specific developmental process. The identification of TFs DNA-targets on a genome-wide scale provides fundamental information not only on how the expression of the targeted genes are regulated but also about the molecular mechanisms behind this process. QsMYB1 is a R2R3-MYB transcription factor related to secondary growth and cork development in Quercus suber. Given the importance of cork quality for the cork-based industry, the study of genes involved in cork formation can provide important insights. In this context, the objective of this PhD thesis was to further study QsMYB1 gene, by identifying its DNA targets on the cork oak genome, in order to elucidate the gene regulatory mechanism in which QsMYB1 exerts its function
Autores principais:Capote, Tiago Costa
Assunto:QsMYB1 R2-R3 transcription factor ChIP-Seq somatic embryogenesis transcriptome
Ano:2018
País:Portugal
Tipo de documento:tese de doutoramento
Tipo de acesso:acesso aberto
Instituição associada:Universidade de Lisboa
Idioma:inglês
Origem:Repositório da Universidade de Lisboa
Descrição
Resumo:The study of transcription factors (TFs) can reveal important players behind a specific developmental process. The identification of TFs DNA-targets on a genome-wide scale provides fundamental information not only on how the expression of the targeted genes are regulated but also about the molecular mechanisms behind this process. QsMYB1 is a R2R3-MYB transcription factor related to secondary growth and cork development in Quercus suber. Given the importance of cork quality for the cork-based industry, the study of genes involved in cork formation can provide important insights. In this context, the objective of this PhD thesis was to further study QsMYB1 gene, by identifying its DNA targets on the cork oak genome, in order to elucidate the gene regulatory mechanism in which QsMYB1 exerts its function