Publicação
Cellular responses to 3D-printed PEEK based implant materials
| Resumo: | Objectives: The aim of this study was to characterize and compare the behavior of human gingival fibroblasts in contact with novel 3D-printed polyetheretherketone (PEEK) implant surface materials, evaluate early bacterial adhesion on these surfaces, and compare its surface roughness. Materials and methods: Four groups of disc-shaped specimens were tested: 1) PEEK printed by fused deposition modelling (FDM-PEEK) with horizontal printing orientation (PH); 2) FDM-PEEK printed vertically (PV), 3) cpTi and 4) ZrO2. Human gingival fibroblasts were cultured on discs (15/group), with their viability evaluated (1, 3 and 7 days) and produced cytokines (IL-6 and IL-8) measured at 3 days. Discs (9/group) also received a Streptococcus oralis suspension, and bacteria were counted at 1 and 2 days. Discs also underwent scanning electron microscopy and surface roughness testing. Groups were compared by ANOVA and Tukey HSD (α=0.05). Results: Although fibroblast proliferation was similar for the groups at 24h (p=0.245), values were significantly higher for PV than ZrO2 at 3 days (p=0.021). At 7 days, PH had the lowest value, being significantly lower than cpTi (p= 0.028). Groups did not influence the production of IL-8 (p=0.095), while IL-6 was higher on cpTi compared to ZrO2 (p=0.015). Bacterial adhesion was lower for ZrO2 than for PH and cpTi at 24h (Kruskal-Wallis p=0.019), but similar at 48h (Kruskal-Wallis p=0.050). PV was significantly rougher than the other groups (p=0.001). Conclusion: The FDM-PEEK produces early fibroblast adhesion and viability as well as bacterial colonization comparable to reference implant materials. Vertical layer printing orientation promoted the best fibroblast adhesion. Clinical significance: This study underscores the potential of FDM-PEEK as a constituent material for dental implants and prosthetic components, with good peri-implant mucosal seal. |
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| Autores principais: | Branco, Rafaela Lopes |
| Assunto: | Teses de mestrado - 2023 Saúde Oral |
| Ano: | 2023 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| Resumo: | Objectives: The aim of this study was to characterize and compare the behavior of human gingival fibroblasts in contact with novel 3D-printed polyetheretherketone (PEEK) implant surface materials, evaluate early bacterial adhesion on these surfaces, and compare its surface roughness. Materials and methods: Four groups of disc-shaped specimens were tested: 1) PEEK printed by fused deposition modelling (FDM-PEEK) with horizontal printing orientation (PH); 2) FDM-PEEK printed vertically (PV), 3) cpTi and 4) ZrO2. Human gingival fibroblasts were cultured on discs (15/group), with their viability evaluated (1, 3 and 7 days) and produced cytokines (IL-6 and IL-8) measured at 3 days. Discs (9/group) also received a Streptococcus oralis suspension, and bacteria were counted at 1 and 2 days. Discs also underwent scanning electron microscopy and surface roughness testing. Groups were compared by ANOVA and Tukey HSD (α=0.05). Results: Although fibroblast proliferation was similar for the groups at 24h (p=0.245), values were significantly higher for PV than ZrO2 at 3 days (p=0.021). At 7 days, PH had the lowest value, being significantly lower than cpTi (p= 0.028). Groups did not influence the production of IL-8 (p=0.095), while IL-6 was higher on cpTi compared to ZrO2 (p=0.015). Bacterial adhesion was lower for ZrO2 than for PH and cpTi at 24h (Kruskal-Wallis p=0.019), but similar at 48h (Kruskal-Wallis p=0.050). PV was significantly rougher than the other groups (p=0.001). Conclusion: The FDM-PEEK produces early fibroblast adhesion and viability as well as bacterial colonization comparable to reference implant materials. Vertical layer printing orientation promoted the best fibroblast adhesion. Clinical significance: This study underscores the potential of FDM-PEEK as a constituent material for dental implants and prosthetic components, with good peri-implant mucosal seal. |
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