Publicação
In vitro evaluation of specific phages against Pseudomonas aeruginosa otitis isolates from dogs
| Resumo: | ABSTRACT - Canine otitis externa (OE) secondarily caused by Pseudomonas aeruginosa is a concerning issue in Veterinary Medicine, due to P. aeruginosa’s high biofilm-forming ability and multidrug resistance profile, being classified by WHO as a critical priority pathogen for the development of new antimicrobials. Considering that, it is mandatory to find innovative antimicrobial strategies, such as bacteriophage therapy. Lytic bacteriophages are viruses able to infect bacteria, with high specificity to their target hosts, making them an excellent choice for therapeutic application, in both Human and Veterinary Medicine. This study aimed to evaluate the inhibitory potential of bacteriophages JG005 and JG024 against P. aeruginosa isolates obtained from dogs with OE (n=49), first characterized with respect to biofilm-forming ability and susceptibility profile to 11 antibiotics. The two bacteriophages were characterized with respect to their titer, host range and activity against P. aeruginosa biofilms. JG024 showed lytic activity against 61.2% (n=30/49) of the isolates, while JG005 showed lytic activity only against 38.8% (n=19/49) of the isolates. The use of Alamar Blue as a method to quantify biofilm suppression by bacteriophages did not show strong microbial suppression, regardless of the phage and multiplicity of infection (MOI) used. Crystal Violet quantification allowed us to observe that JG005 showed strong microbial suppression for 60% (n=6/10) of the isolates when applied at MOI 10, and for 30% (n=3/10) of the isolates at MOI 100. JG024 showed strong microbial suppression for 10% (n=1/10) of the isolates regardless of the MOI tested. These phages showed interesting results in the present study and may be considered an innovative alternative with great potential for the treatment and prevention of OE by P. aeruginosa in the dog, suggesting the need to continue this line of research with the goal of its future use in clinical settings |
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| Autores principais: | Lourenço, Maura Helena Ribeiro |
| Assunto: | Bacteriophage Pseudomonas aeruginosa Otitis externa Dog Biofilm Bacteriófago Pseudomonas aeruginosa Otite externa Cão Biofilme |
| Ano: | 2023 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| Resumo: | ABSTRACT - Canine otitis externa (OE) secondarily caused by Pseudomonas aeruginosa is a concerning issue in Veterinary Medicine, due to P. aeruginosa’s high biofilm-forming ability and multidrug resistance profile, being classified by WHO as a critical priority pathogen for the development of new antimicrobials. Considering that, it is mandatory to find innovative antimicrobial strategies, such as bacteriophage therapy. Lytic bacteriophages are viruses able to infect bacteria, with high specificity to their target hosts, making them an excellent choice for therapeutic application, in both Human and Veterinary Medicine. This study aimed to evaluate the inhibitory potential of bacteriophages JG005 and JG024 against P. aeruginosa isolates obtained from dogs with OE (n=49), first characterized with respect to biofilm-forming ability and susceptibility profile to 11 antibiotics. The two bacteriophages were characterized with respect to their titer, host range and activity against P. aeruginosa biofilms. JG024 showed lytic activity against 61.2% (n=30/49) of the isolates, while JG005 showed lytic activity only against 38.8% (n=19/49) of the isolates. The use of Alamar Blue as a method to quantify biofilm suppression by bacteriophages did not show strong microbial suppression, regardless of the phage and multiplicity of infection (MOI) used. Crystal Violet quantification allowed us to observe that JG005 showed strong microbial suppression for 60% (n=6/10) of the isolates when applied at MOI 10, and for 30% (n=3/10) of the isolates at MOI 100. JG024 showed strong microbial suppression for 10% (n=1/10) of the isolates regardless of the MOI tested. These phages showed interesting results in the present study and may be considered an innovative alternative with great potential for the treatment and prevention of OE by P. aeruginosa in the dog, suggesting the need to continue this line of research with the goal of its future use in clinical settings |
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