Publicação
Synergism between antibiotics and natural plant extracts : tests of antibacterial activity by natural compounds extracted from czech domestic plants
| Resumo: | For many years, antimicrobial drugs, such as antibiotics, have been successfully used to treat patients with bacterial and infectious diseases. In the recent years, however, many microbes have adapted in an effort to evade to the drugs designed to destroy or inactivate them. The evolution of antibiotic resistance is nowadays of great concern to the global health community. New resistance mechanisms emerge rapidly and spread globally threatening our ability to effectively treat disease. One potential source of novel drugs is traditional medicine and extracts from herbs. This study explores the potential of several plants harvested in Czech Republic and screened for antibacterial activity. After identification and harvesting, a total of 80 different aqueous extracts were prepared from 38 different herbal species. Antibacterial activity against Salmonella typhimurium microbes was inferred through replicate disc diffusion assays and synergy with four antibiotics - ampicillin (AMP), chloramphenicol (CLP), streptomycin (STRP) and tetracycline (TETR) - was tested. Even though the analysis was very preliminary, four plants exhibited major synergistic antibacterial activity: Sanguisorba officinalis, Lysimachia punctata, Rhus typhina and Paris quadrifolia. This data suggests that further screening of plants is warranted and we put forward several species for further investigation of activity against S. typhimurium. |
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| Autores principais: | Rodrigues, Bruna Filipa |
| Assunto: | Mestrado Integrado - 2014 |
| Ano: | 2014 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| Resumo: | For many years, antimicrobial drugs, such as antibiotics, have been successfully used to treat patients with bacterial and infectious diseases. In the recent years, however, many microbes have adapted in an effort to evade to the drugs designed to destroy or inactivate them. The evolution of antibiotic resistance is nowadays of great concern to the global health community. New resistance mechanisms emerge rapidly and spread globally threatening our ability to effectively treat disease. One potential source of novel drugs is traditional medicine and extracts from herbs. This study explores the potential of several plants harvested in Czech Republic and screened for antibacterial activity. After identification and harvesting, a total of 80 different aqueous extracts were prepared from 38 different herbal species. Antibacterial activity against Salmonella typhimurium microbes was inferred through replicate disc diffusion assays and synergy with four antibiotics - ampicillin (AMP), chloramphenicol (CLP), streptomycin (STRP) and tetracycline (TETR) - was tested. Even though the analysis was very preliminary, four plants exhibited major synergistic antibacterial activity: Sanguisorba officinalis, Lysimachia punctata, Rhus typhina and Paris quadrifolia. This data suggests that further screening of plants is warranted and we put forward several species for further investigation of activity against S. typhimurium. |
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