Publicação
The role of Mesogenin1 in the dynamics of movement and differentiation of mesoderm progenitor cells
| Resumo: | Gastrulation in zebrafish involves four cell movements: epiboly, involution, convergence and extension. These cell movements are required to move the mesoderm progenitor cells (MPCs) located at the lateral region of the germ ring to the anterior segmental plate so that they can give rise to trunk somites. The tailbud, which is formed at the end of gastrulation, contains the MPCs that will give rise to tail somites and therefore, at this location, their movements into the posterior presomitic mesoderm (PSM) also need to be tightly controlled. Work from our lab has shown that the transcription factor Mesogenin1 (Msgn1) regulates cell movements in the tailbud. Since msgn1 is expressed not only in the tailbud during somitogenesis, but also in the germ ring, it could also control cell movements during gastrulation. To analyze the role of Msgn1 on cell movements, a cell co-transplantation technique was performed using wild-type cells and mutant or transgenic cells from a msgn1-/- line or a hsp70:HA-msgn1 line that allows time-specific over-expression of msgn1, respectively. The results suggest that Msgn1 is involved in the regulation of convergence and extension movements during gastrulation and confirm that it is also involved in governing the flux of cells from the tailbud into the PSM. Finally, to determine if msgn1 induces differentiation of MPCs, and based on a microarray experiment previously performed in our lab, the expression patterns of two target genes that are important for paraxial mesoderm production were analyzed: crabp2b and ripply1. The results indicate that Msgn1 induces their expression, suggesting that it also contributes to switch-on the PSM character. Thus, Mesogenin1 plays a key role during development, coupling MPCs movements during gastrulation and tail extension with the acquisition of a PSM fate. |
|---|---|
| Autores principais: | Figueira, Ana Margarida da Silva, 1990- |
| Assunto: | Peixe-zebra Embriologia animal Hibridação in situ Teses de mestrado - 2013 |
| Ano: | 2013 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| Resumo: | Gastrulation in zebrafish involves four cell movements: epiboly, involution, convergence and extension. These cell movements are required to move the mesoderm progenitor cells (MPCs) located at the lateral region of the germ ring to the anterior segmental plate so that they can give rise to trunk somites. The tailbud, which is formed at the end of gastrulation, contains the MPCs that will give rise to tail somites and therefore, at this location, their movements into the posterior presomitic mesoderm (PSM) also need to be tightly controlled. Work from our lab has shown that the transcription factor Mesogenin1 (Msgn1) regulates cell movements in the tailbud. Since msgn1 is expressed not only in the tailbud during somitogenesis, but also in the germ ring, it could also control cell movements during gastrulation. To analyze the role of Msgn1 on cell movements, a cell co-transplantation technique was performed using wild-type cells and mutant or transgenic cells from a msgn1-/- line or a hsp70:HA-msgn1 line that allows time-specific over-expression of msgn1, respectively. The results suggest that Msgn1 is involved in the regulation of convergence and extension movements during gastrulation and confirm that it is also involved in governing the flux of cells from the tailbud into the PSM. Finally, to determine if msgn1 induces differentiation of MPCs, and based on a microarray experiment previously performed in our lab, the expression patterns of two target genes that are important for paraxial mesoderm production were analyzed: crabp2b and ripply1. The results indicate that Msgn1 induces their expression, suggesting that it also contributes to switch-on the PSM character. Thus, Mesogenin1 plays a key role during development, coupling MPCs movements during gastrulation and tail extension with the acquisition of a PSM fate. |
|---|