Publicação
Searching for potencial antibiotic agents of natural and synthetic origin and their interaction with amyloid fibril formation systems
| Resumo: | New molecules from synthetic and natural origin, capable of dealing with conditions concerning ageing and/or infection, were investigated by the following approaches: Synthesis of non-toxic alkyl 2,6-deoxy glycosides with potential antibiotic and anti-amyloidogenic activity Identification of active principles of S. sclareoides regarding its anti-amyloidogenic effects.Infections are frequently more severe in elderly population and antibiotics endorsing active and healthier ageing become relevant. Antimicrobial activity was assessed by microdilution method, and the anti-amyloidogenic activity was assessed by fibrilisation/defibrilisation of cystatin B, monitored with ThT and fluorescence spectroscopy. Moreover, plant extracts, their pure components and synthetic glycosides were added to 15N cystatin B and HSQC spectra showed binding to the protein in loop residues and β strands. The ability of synthetic compounds, extracts and pure components to prevent conversion of PrPC to PrPSc was measured by dot blot analysis. The results obtained reinforce the potential of some of the tested compounds/extracts for drug research in both ageing and infection. |
|---|---|
| Autores principais: | Dias, Catarina Alexandra dos Santos |
| Assunto: | Bacillus Carbohydrate based antibiotics Neuroprotectors S. sclareoides Amyloid fibrils Rosmarinic acid Teses de mestrado - 2012 |
| Ano: | 2012 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| Resumo: | New molecules from synthetic and natural origin, capable of dealing with conditions concerning ageing and/or infection, were investigated by the following approaches: Synthesis of non-toxic alkyl 2,6-deoxy glycosides with potential antibiotic and anti-amyloidogenic activity Identification of active principles of S. sclareoides regarding its anti-amyloidogenic effects.Infections are frequently more severe in elderly population and antibiotics endorsing active and healthier ageing become relevant. Antimicrobial activity was assessed by microdilution method, and the anti-amyloidogenic activity was assessed by fibrilisation/defibrilisation of cystatin B, monitored with ThT and fluorescence spectroscopy. Moreover, plant extracts, their pure components and synthetic glycosides were added to 15N cystatin B and HSQC spectra showed binding to the protein in loop residues and β strands. The ability of synthetic compounds, extracts and pure components to prevent conversion of PrPC to PrPSc was measured by dot blot analysis. The results obtained reinforce the potential of some of the tested compounds/extracts for drug research in both ageing and infection. |
|---|