Publicação
Incorporation of silica-coated superparamagnetic nanoparticles clusters in liposomes by thin film method
| Resumo: | Superparamagnetic iron oxide nanoparticles (SPIONs) are recognized to be an attractive platform for developing novel drug delivery approaches, they also have attracted attention because of their potential usefulness as contrast agents for magnetic resonance imaging (MRI) or as colloidal mediators for cancer magnetic hyperthermia. In the present work, we have successfully incorporated silica-coated superparamagnetic maghemite (gamma-Fe2O3) nanoparticles clusters (SMCs) in liposomes by thin film method. Preliminary trials were conducted in order to optimize the preparation method, the SMCs percentage in the formulation and the lipid composition of the magnetoliposomes. Different magnetoliposomes having bilayer membranes composed of soybean phosphatidylcholine, hydrogenated soybean phosphatidylcholine and cholesterol were synthesised. The influence of two stabilizers – protasan and poloxamer 188 – on the stability of magnetoliposomes was also evaluated. After synthesis, the samples were characterized through Dynamic Light Scattering and some by Transmission Electron Microscopy. The results were satisfactory, with TEM and DLS defining the structure of the magnetoliposomes, showing the distribution of the SMCs in the samples and allowing us to infer about the systems stability. |
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| Autores principais: | Pissarra, Ana Margarida de Castro e Vasconcelos |
| Assunto: | SPION Drug delivery MRI Hyperthermia SMC Magnetoliposomes Stabilizer TEM DLS Mestrado Integrado - 2014 |
| Ano: | 2014 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| Resumo: | Superparamagnetic iron oxide nanoparticles (SPIONs) are recognized to be an attractive platform for developing novel drug delivery approaches, they also have attracted attention because of their potential usefulness as contrast agents for magnetic resonance imaging (MRI) or as colloidal mediators for cancer magnetic hyperthermia. In the present work, we have successfully incorporated silica-coated superparamagnetic maghemite (gamma-Fe2O3) nanoparticles clusters (SMCs) in liposomes by thin film method. Preliminary trials were conducted in order to optimize the preparation method, the SMCs percentage in the formulation and the lipid composition of the magnetoliposomes. Different magnetoliposomes having bilayer membranes composed of soybean phosphatidylcholine, hydrogenated soybean phosphatidylcholine and cholesterol were synthesised. The influence of two stabilizers – protasan and poloxamer 188 – on the stability of magnetoliposomes was also evaluated. After synthesis, the samples were characterized through Dynamic Light Scattering and some by Transmission Electron Microscopy. The results were satisfactory, with TEM and DLS defining the structure of the magnetoliposomes, showing the distribution of the SMCs in the samples and allowing us to infer about the systems stability. |
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