Publicação
Effects of salivary acetylcholinesterase on the cytotoxicity of acrylic reline resins
| Resumo: | The use of autopolymerizing acrylic reline resins has recently gained popularity to readapt dentures to the continuous reabsorbed underlying tissues. However, these materials have been associated with higher levels of toxicity in vitro, and chemical irritation and allergic reactions in vivo. These biomaterials are subject of degradation in the oral cavity, in which enzymatic activity oh hydrolases plays an important role, particularly the acethylcholinesterase enzyme. The main objective of this study is to assess the effect of a salivary esterase on the cytotoxicity of three acrylic reline resins, two directs, Kooliner and Ufi Gel Hard, and one indirect, Probase Cold, using two colorimetric functional assays, MTT and LDH. This work will try to assess the cytotoxicity of the monomers using pure compounds of IBMA, HDMA, MMA and their common by-product, MA, taking into account the IC50. The IC50 of the acrylic reline resin liquids was also studied. In this study, the exposure of fibroblasts to direct reline resins, Kooliner and Ufi Gel Hard, eluates resulted in a significant suppression of fibroblastic function, characterized by supressed mitochondrial activity. On the contrary, Probase Cold eluate didin’t exhibit cytotoxic activity. The LDH a ssay was found to be less sensitive that the MTT assay when assessing the cytotoxic effect of the evaluated materials. Cells incubated with eluates treated with acethylcholinesterase changed their response to eluates from direct reline resins. The experimental specimens revealed an increase of cell viability. The non-cytotoxic effect of Probase Cold didn’t change. No cytotoxic effects were observed with the monomers, at the concentrations found to be leached in the oral cavity, when exposed to human primary fibroblasts. Considering the IC50 of the residual monomers, the cytotoxicity decreased in order of HDMA>IBMA>MA. MMA showed no biologic effect at the concentrations used. |
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| Autores principais: | Oliveira, Miguel Constantino Mendes de |
| Assunto: | Materiais dentários Teses de mestrado - 2013 |
| Ano: | 2013 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| Resumo: | The use of autopolymerizing acrylic reline resins has recently gained popularity to readapt dentures to the continuous reabsorbed underlying tissues. However, these materials have been associated with higher levels of toxicity in vitro, and chemical irritation and allergic reactions in vivo. These biomaterials are subject of degradation in the oral cavity, in which enzymatic activity oh hydrolases plays an important role, particularly the acethylcholinesterase enzyme. The main objective of this study is to assess the effect of a salivary esterase on the cytotoxicity of three acrylic reline resins, two directs, Kooliner and Ufi Gel Hard, and one indirect, Probase Cold, using two colorimetric functional assays, MTT and LDH. This work will try to assess the cytotoxicity of the monomers using pure compounds of IBMA, HDMA, MMA and their common by-product, MA, taking into account the IC50. The IC50 of the acrylic reline resin liquids was also studied. In this study, the exposure of fibroblasts to direct reline resins, Kooliner and Ufi Gel Hard, eluates resulted in a significant suppression of fibroblastic function, characterized by supressed mitochondrial activity. On the contrary, Probase Cold eluate didin’t exhibit cytotoxic activity. The LDH a ssay was found to be less sensitive that the MTT assay when assessing the cytotoxic effect of the evaluated materials. Cells incubated with eluates treated with acethylcholinesterase changed their response to eluates from direct reline resins. The experimental specimens revealed an increase of cell viability. The non-cytotoxic effect of Probase Cold didn’t change. No cytotoxic effects were observed with the monomers, at the concentrations found to be leached in the oral cavity, when exposed to human primary fibroblasts. Considering the IC50 of the residual monomers, the cytotoxicity decreased in order of HDMA>IBMA>MA. MMA showed no biologic effect at the concentrations used. |
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