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Estudo comparativo de métodos de avaliação da capacidade antioxidante de compostos bioactivos

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Resumo:The objectives of this study concern in evaluate the antioxidant capability of potentially rich matrices. The antioxidant activity of the oregano (Origanum virens L.), essential oregano oil resulting from the hydro distillation and Soxhlet extraction, lyophilized tomato industrial waste, olive industrial waste and natural and commercial blueberry juice was evaluated. The study was started by applying the Rancimat method to oregano and its extracts observing the use of several extracts as well as its evolution through time. The Quencher method based on radical scavenging ability, allows the evolution of antioxidant ability on solids matrices avoiding by this way extraction procedures. , therefore obtaining results that are closer to reality. These methods were applied to various natural matrices. It was concluded that Quencher method allows a more rapid assessment of the activity of the compounds, but is influenced by the nature of the solid matrix. From results we can conclude that whole oregano presents a greater antioxidant power than any of its extracts, tomato and olive by-products. We can also conclude that natural blueberry juice is richer in antioxidants than commercial blueberry juice.
Autores principais:Pereira, Mariana Oliveira de Sousa
Assunto:Origanum virens L. extract antioxidant activity Rancimat Quencher
Ano:2010
País:Portugal
Tipo de documento:dissertação de mestrado
Tipo de acesso:acesso aberto
Instituição associada:Universidade de Lisboa
Idioma:português
Origem:Repositório da Universidade de Lisboa
Descrição
Resumo:The objectives of this study concern in evaluate the antioxidant capability of potentially rich matrices. The antioxidant activity of the oregano (Origanum virens L.), essential oregano oil resulting from the hydro distillation and Soxhlet extraction, lyophilized tomato industrial waste, olive industrial waste and natural and commercial blueberry juice was evaluated. The study was started by applying the Rancimat method to oregano and its extracts observing the use of several extracts as well as its evolution through time. The Quencher method based on radical scavenging ability, allows the evolution of antioxidant ability on solids matrices avoiding by this way extraction procedures. , therefore obtaining results that are closer to reality. These methods were applied to various natural matrices. It was concluded that Quencher method allows a more rapid assessment of the activity of the compounds, but is influenced by the nature of the solid matrix. From results we can conclude that whole oregano presents a greater antioxidant power than any of its extracts, tomato and olive by-products. We can also conclude that natural blueberry juice is richer in antioxidants than commercial blueberry juice.