Publicação
Reprogramming of fibroblasts intoinduced Pluripotent Stem Cells – iPSC : hepatitis C – challenges and progress
| Resumo: | Hepatitis C is a liver disease that results from an infection by the Hepatitis C virus. It can range between severe to moderate illness, with variable time. Today, the most infected people are associate with drugs and injectable material. Until 1992, an individual was mostly infected by blood transfusions or organ transplants. This work reflects, therefore, the attempt to develop different strategies to the detailed study of hepatitis C virus, using a novel cellular strategy. The aim of the study is to obtain the iPSCs from fibroblasts by infected patients with the virus. Subsequently, it will be made differentiate into hepatocytes like cells. This development happens through progressive steps of cellular manipulation with many different conditions, culture mediums and techniques. Then, these cells are used to viral broader study, with results that mimic liver human conditions. In parallel, it was also tested forty five plasma samples from infected patients to comparing infection levels of different HCV genotypes as well as the response to drug therapy. The results were satisfactory once it was accomplished iPSC production, and it was possible to obtain conclusions with the RNA analysis. |
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| Autores principais: | Oliveira, Catarina Alexandra Grilo de |
| Assunto: | iPSC HCV Reprogramming Viral RNA Mestrado Integrado - 2013 |
| Ano: | 2013 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| Resumo: | Hepatitis C is a liver disease that results from an infection by the Hepatitis C virus. It can range between severe to moderate illness, with variable time. Today, the most infected people are associate with drugs and injectable material. Until 1992, an individual was mostly infected by blood transfusions or organ transplants. This work reflects, therefore, the attempt to develop different strategies to the detailed study of hepatitis C virus, using a novel cellular strategy. The aim of the study is to obtain the iPSCs from fibroblasts by infected patients with the virus. Subsequently, it will be made differentiate into hepatocytes like cells. This development happens through progressive steps of cellular manipulation with many different conditions, culture mediums and techniques. Then, these cells are used to viral broader study, with results that mimic liver human conditions. In parallel, it was also tested forty five plasma samples from infected patients to comparing infection levels of different HCV genotypes as well as the response to drug therapy. The results were satisfactory once it was accomplished iPSC production, and it was possible to obtain conclusions with the RNA analysis. |
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