Publicação
Cellular responses to 3D printed dental resins produced using consumer versus High-End printers
| Resumo: | Objectives: The aim of this study was to evaluate the influence of different 3D dental printers and resins on human gingival fibroblasts behaviour. Materials and Methods: Three resins from NextDent (Denture 3D+, C&B MFH and Crowntec) were used to produce disc-shaped specimens on NextDent 5100 and Phrozen Sonic Mini 4K printers, using equivalent parameters in a total of 6 groups (N=20), with groups PD, PC and PT corresponding to Denture 3D+, C&B MFH and Crowntec printed on Phrozen printer, respectively and ND, NC and NT corresponding to Denture 3D+, C&B MFH and Crowntec printed on NexDent printer, respectively . Human gingival fibroblasts were cultured on specimens and biocompatibility evaluated at 1,3 and 7 days. IL-6 and IL-8 concentrations were evaluated at 3 days of culture using ELISA. Surface roughness was evaluated by a contact profilometer. SEM and fluorescence micrographs were analyzed at 1 and 7 days of growth. Statistical analyses were performed using SPSS 28.0 version and mean differences were tested using ANOVA and post-hoc Tukey tests (p< 0.05). Results: There was an increase in cellular growth after 7 days in culture in group PC and in group PT when compared to group PD (p=0,028 and p=0,203, respectively). ND group resulted in higher concentration of IL-6 when compared to PT group, and NC resulted in higher concentration of IL-8 when compared to ND, NT, PD and PT groups. No significant differences were found between groups regarding surface roughness. Conclusions: Within the limitations of the present study, NextDent 5100 and Phrozen Sonic Mini 4K performed similarly considering cell responses. The use of different 3D-printing resins influences in-vitro cellular behaviour of human fibroblasts. Surface roughness did not seem to be influenced using different printers or resins, if equivalent parameters are used. |
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| Autores principais: | Cardoso, Beatriz Sona |
| Assunto: | Teses de mestrado - 2023 Saúde Oral |
| Ano: | 2023 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | português |
| Origem: | Repositório da Universidade de Lisboa |
| Resumo: | Objectives: The aim of this study was to evaluate the influence of different 3D dental printers and resins on human gingival fibroblasts behaviour. Materials and Methods: Three resins from NextDent (Denture 3D+, C&B MFH and Crowntec) were used to produce disc-shaped specimens on NextDent 5100 and Phrozen Sonic Mini 4K printers, using equivalent parameters in a total of 6 groups (N=20), with groups PD, PC and PT corresponding to Denture 3D+, C&B MFH and Crowntec printed on Phrozen printer, respectively and ND, NC and NT corresponding to Denture 3D+, C&B MFH and Crowntec printed on NexDent printer, respectively . Human gingival fibroblasts were cultured on specimens and biocompatibility evaluated at 1,3 and 7 days. IL-6 and IL-8 concentrations were evaluated at 3 days of culture using ELISA. Surface roughness was evaluated by a contact profilometer. SEM and fluorescence micrographs were analyzed at 1 and 7 days of growth. Statistical analyses were performed using SPSS 28.0 version and mean differences were tested using ANOVA and post-hoc Tukey tests (p< 0.05). Results: There was an increase in cellular growth after 7 days in culture in group PC and in group PT when compared to group PD (p=0,028 and p=0,203, respectively). ND group resulted in higher concentration of IL-6 when compared to PT group, and NC resulted in higher concentration of IL-8 when compared to ND, NT, PD and PT groups. No significant differences were found between groups regarding surface roughness. Conclusions: Within the limitations of the present study, NextDent 5100 and Phrozen Sonic Mini 4K performed similarly considering cell responses. The use of different 3D-printing resins influences in-vitro cellular behaviour of human fibroblasts. Surface roughness did not seem to be influenced using different printers or resins, if equivalent parameters are used. |
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