Publicação
Population analysis of Hyla arborea and Hyla meridionalis (Amphibia, Anura) in Portugal:a molecular genetic and bioacoustic approach
| Resumo: | The focus of this work is on the species of Hylids (Amphibia, Anura) present in Portugal, Hyla meridionalis and Hyla arborea (=H. molleri), to examine the geographic patterns of genetic and mating calls diversity. The aim is to determine the patterns of variation of genetic and bioacoustic markers between populations, exploring how the current distribution patterns were influenced by the origins, colonization events of both species, and finally their evolutive history. For comparison and biogeographically integrative reasons, information from outside Portugal is used. The genetic and bioacoustic analyses revealed different diversity patterns among the studied populations of both species. Iberian H. arborea is highly divergent from European taxon, supporting the ressurection of H. molleri species. Within Iberia two distinct groups, ‘North’ and ‘South’, were identified, having the southern one higher haplotype diversity, a pattern seen in other Iberian amphibians. A different pattern was however seen at the bioacoustic level. Populations did not cluster into groups, and no significant differences were found between populations. In H. meridionalis COI analysis corroborated previous results, with three well differentiated clades identified, all with little genetic diversity within groups. Tunisia and Algeria specimens were highly divergent from all other, suggesting an ancient split between the two lineages. Advertisement calls showed little diversity between populations. In syntopy, the presence of males of the other species calling apparently has little effect on advertisement calls: only frequencies and call duration of H. meridionalis were significantly smaller in sympatry. Being unlikely the hypothesis of reproductive character displacement of mating calls. This, together with the absence of F1 hybrids in the studied sites (although earlier studies have reported their presence) and of any sign of introgression in adults and tadpoles, suggests a rare occurrence of heterospecific mating, and that advertisement calls are an effective premating barrier. |
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| Autores principais: | Moreira, Catarina Nascimento, 1978- |
| Assunto: | Hyla arborea Hyla meridionalis Genética Bioacústica Teses de doutoramento - 2013 |
| Ano: | 2013 |
| País: | Portugal |
| Tipo de documento: | tese de doutoramento |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| Resumo: | The focus of this work is on the species of Hylids (Amphibia, Anura) present in Portugal, Hyla meridionalis and Hyla arborea (=H. molleri), to examine the geographic patterns of genetic and mating calls diversity. The aim is to determine the patterns of variation of genetic and bioacoustic markers between populations, exploring how the current distribution patterns were influenced by the origins, colonization events of both species, and finally their evolutive history. For comparison and biogeographically integrative reasons, information from outside Portugal is used. The genetic and bioacoustic analyses revealed different diversity patterns among the studied populations of both species. Iberian H. arborea is highly divergent from European taxon, supporting the ressurection of H. molleri species. Within Iberia two distinct groups, ‘North’ and ‘South’, were identified, having the southern one higher haplotype diversity, a pattern seen in other Iberian amphibians. A different pattern was however seen at the bioacoustic level. Populations did not cluster into groups, and no significant differences were found between populations. In H. meridionalis COI analysis corroborated previous results, with three well differentiated clades identified, all with little genetic diversity within groups. Tunisia and Algeria specimens were highly divergent from all other, suggesting an ancient split between the two lineages. Advertisement calls showed little diversity between populations. In syntopy, the presence of males of the other species calling apparently has little effect on advertisement calls: only frequencies and call duration of H. meridionalis were significantly smaller in sympatry. Being unlikely the hypothesis of reproductive character displacement of mating calls. This, together with the absence of F1 hybrids in the studied sites (although earlier studies have reported their presence) and of any sign of introgression in adults and tadpoles, suggests a rare occurrence of heterospecific mating, and that advertisement calls are an effective premating barrier. |
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