Publicação
Molecular diagnosis of Mycoplasma bovis
| Resumo: | Mycoplasma bovis is a bacteria responsible for different disease presentations in cattle, such as pneumonia, mastitis, otitis, genital disorders, keratoconjuntivitis and arthritis, presently considered as one of the major emerging pathogens affecting cattle. Until this day, it is responsible for losses in animal production of over 150 million euros across Europe. The pathogenesis of Mycoplasma-associated diseases is multifactorial and the highly variable surface lipoproteins allows a fast and efficient dissemination of M. bovis within the host and the herd. Due to its high antigenic plasticity, its ability to survive within multiple host cells and the capacity to establish multiple synergistic interactions with other pathogens, makes M. bovis and associated infections are a major challenge in Veterinary Medicine, since the vaccine is not efficient and antibiotics are almost inefficient. This study aims at developing and validating a quantitative PCR protocol for the diagnosis of M. bovis. 93 milk samples, from 5 different Portuguese farms, were collected, processed and each one’s DNA extracted to be analyzed through a qPCR method targeting the uvrC and uvrC2024 genes. Given the percentage of positivity, which was high, the conclusion we can take from the study is that there is still work to do, in terms of establishing a uniformed practice to tackle the wide presence of M. bovis in farms. |
|---|---|
| Autores principais: | Gonçalves, António Maria Nolasco Cordeiro Gomes |
| Assunto: | Mycoplasma bovis qPCR Diagnosis Mastitis Milk Mycoplasma bovis qPCR Diagnóstico Mastite Leite |
| Ano: | 2022 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| Resumo: | Mycoplasma bovis is a bacteria responsible for different disease presentations in cattle, such as pneumonia, mastitis, otitis, genital disorders, keratoconjuntivitis and arthritis, presently considered as one of the major emerging pathogens affecting cattle. Until this day, it is responsible for losses in animal production of over 150 million euros across Europe. The pathogenesis of Mycoplasma-associated diseases is multifactorial and the highly variable surface lipoproteins allows a fast and efficient dissemination of M. bovis within the host and the herd. Due to its high antigenic plasticity, its ability to survive within multiple host cells and the capacity to establish multiple synergistic interactions with other pathogens, makes M. bovis and associated infections are a major challenge in Veterinary Medicine, since the vaccine is not efficient and antibiotics are almost inefficient. This study aims at developing and validating a quantitative PCR protocol for the diagnosis of M. bovis. 93 milk samples, from 5 different Portuguese farms, were collected, processed and each one’s DNA extracted to be analyzed through a qPCR method targeting the uvrC and uvrC2024 genes. Given the percentage of positivity, which was high, the conclusion we can take from the study is that there is still work to do, in terms of establishing a uniformed practice to tackle the wide presence of M. bovis in farms. |
|---|