Publicação
Fabrication of electrochemical biosensors for the detection of glucose prepared by covalent immobilization of glucose oxidase on conducting (poly)thiophene films
| Resumo: | Biochemical biosensors have emerged as important analytical tools with application in diverse fields and biosensors for the detection of glucose using the enzyme glucose oxidase have been widely investigated. In this work the preparation of biosensors by electrochemical polymerization of (poly)thiophenes, namely 2,2’-bithiophene, 2,2’-bithiophene and thiophene-3-carboxylic acid, and 4,4’-bis(2-methyl-3-butin-2-ol)-2,2’-bithiophene, followed by covalent immobilization of glucose oxidase on the films, using N-Cyclohexyl-N’-(2-morpholinoethyl)carbodiimide metho-p-toluenesulfonate as a condensing agent, is described. The glucose oxidase electrodes with films of 2,2’-bithiophene and 4,4’-bis(2-methyl-3-butin-2-ol)-2,2’-bithiophene were then tested for their ability in detecting glucose from simple aqueous solutions of glucose and from real samples of fruit juices (pear, apricot and peach). p-benzoquinone was used as a mediator in solution. Being this a preliminary study made in a short period of time, the results accomplished were considered very positive. |
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| Autores principais: | Sobral, Ana Isabel Figueiredo |
| Assunto: | Mestrado Integrado - 2014 |
| Ano: | 2014 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| Resumo: | Biochemical biosensors have emerged as important analytical tools with application in diverse fields and biosensors for the detection of glucose using the enzyme glucose oxidase have been widely investigated. In this work the preparation of biosensors by electrochemical polymerization of (poly)thiophenes, namely 2,2’-bithiophene, 2,2’-bithiophene and thiophene-3-carboxylic acid, and 4,4’-bis(2-methyl-3-butin-2-ol)-2,2’-bithiophene, followed by covalent immobilization of glucose oxidase on the films, using N-Cyclohexyl-N’-(2-morpholinoethyl)carbodiimide metho-p-toluenesulfonate as a condensing agent, is described. The glucose oxidase electrodes with films of 2,2’-bithiophene and 4,4’-bis(2-methyl-3-butin-2-ol)-2,2’-bithiophene were then tested for their ability in detecting glucose from simple aqueous solutions of glucose and from real samples of fruit juices (pear, apricot and peach). p-benzoquinone was used as a mediator in solution. Being this a preliminary study made in a short period of time, the results accomplished were considered very positive. |
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