Publicação
Integração de fungicidas sintéticos e não sintéticos para a redução de perdas pós-colheita em pera Rocha
| Resumo: | The majority of postharvest diseases in pome fruits is caused by fungi. The purpose of this work was to analyse alternatives to synthetic fungicides, to reduce or eliminate its use. Fungal plant pathogens Stemphylium sp., Botrytis cinerea and Penicillium expansum were isolated from infected “Rocha” pear and in vitro antagonistic activity of Aureobasidium pullulans was evaluated. The susceptibility of the antagonist and the pathogenic fungi, to synthetic fungicides (imazalil and thiabendazole) and to chitosan, was also evaluated. The assays were conducted in liquid media (pear broth and PDB), and in these solid media were used the disk-diffusion test and incorporation method, for determination of minimum inhibitory concentration (MIC). A. pullulans showed antagonistic activity regarding all three pathogens in PDA but not in pear agar. In solid media, thiabendazole inhibited the growth of antagonist and B. cinerea. Chitosan (15000 μg/mL) inhibited Stemphylium sp. and B. cinerea. Imazalil allowed inhibition of P. expansum, using product concentrations tolerated by A. pullulans (322,2 μg/mL) and lower than the in use concentration (5155 μg/mL). These results suggest an evaluation of preharvest applications of antagonist, and postharvest applications of imazalil and chitosan, using concentrations tolerated by antagonist. |
|---|---|
| Autores principais: | Nascimento, Filipa Margarida Bulha |
| Assunto: | Aureobasidium pullulans pathogenic fungi imazalil thiabendazole chitosan |
| Ano: | 2015 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | português |
| Origem: | Repositório da Universidade de Lisboa |
| Resumo: | The majority of postharvest diseases in pome fruits is caused by fungi. The purpose of this work was to analyse alternatives to synthetic fungicides, to reduce or eliminate its use. Fungal plant pathogens Stemphylium sp., Botrytis cinerea and Penicillium expansum were isolated from infected “Rocha” pear and in vitro antagonistic activity of Aureobasidium pullulans was evaluated. The susceptibility of the antagonist and the pathogenic fungi, to synthetic fungicides (imazalil and thiabendazole) and to chitosan, was also evaluated. The assays were conducted in liquid media (pear broth and PDB), and in these solid media were used the disk-diffusion test and incorporation method, for determination of minimum inhibitory concentration (MIC). A. pullulans showed antagonistic activity regarding all three pathogens in PDA but not in pear agar. In solid media, thiabendazole inhibited the growth of antagonist and B. cinerea. Chitosan (15000 μg/mL) inhibited Stemphylium sp. and B. cinerea. Imazalil allowed inhibition of P. expansum, using product concentrations tolerated by A. pullulans (322,2 μg/mL) and lower than the in use concentration (5155 μg/mL). These results suggest an evaluation of preharvest applications of antagonist, and postharvest applications of imazalil and chitosan, using concentrations tolerated by antagonist. |
|---|