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Bioactive phenolic compounds action in anti-proliferative and anti-inflammatory activity

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Detalhes bibliográficos
Resumo:This study is focused on three main flavonoids compounds: Kaempferol, which is isolated from several natural plants, it is a polyphenol belonging to the subgroup of flavonoids, it exhibits various pharmacological activities, including anti-inflammatory, antioxidant, antimicrobial, and anticancer activities; Quercetin which is a polyphenolic flavonoid with potential chemopreventive activity, ubiquitous in plant food sources and a major bioflavonoid in the human diet, it also produces anti-inflammatory and anti-allergy effects mediated through the inhibition of the lipoxygenase and cyclooxygenase pathways, thereby preventing the production of pro-inflammatory mediators and Eriodictyol which is a flavonoid with anti-inflammatory and antioxidant activities. Because inflammation and oxidative stress play critical roles in the pathogenesis of diabetes mellitus, the present study was designed to explore whether Eriodictyol, Quercetin and Kaempferol have therapeutic potential for the treatment of common diseases. In this study we studied the anti-proliferative effect of the three flavonoids using RAW 246.7 cells as in vitro model, and observed a dose-dependent reduction in cell viability. We also evaluated the capacity of these flavonoids to inhibit, in vitro, the activity of selected enzymes to assess their potential use as anti-aging, neuroprotective and anti-diabetic agents.
Autores principais:Morais, Adriano José Sousa
Assunto:Cell Biology Biomedical applications
Ano:2020
País:Portugal
Tipo de documento:dissertação de mestrado
Tipo de acesso:acesso aberto
Instituição associada:Universidade de Trás-os-Montes e Alto Douro
Idioma:inglês
Origem:Repositório da UTAD
Descrição
Resumo:This study is focused on three main flavonoids compounds: Kaempferol, which is isolated from several natural plants, it is a polyphenol belonging to the subgroup of flavonoids, it exhibits various pharmacological activities, including anti-inflammatory, antioxidant, antimicrobial, and anticancer activities; Quercetin which is a polyphenolic flavonoid with potential chemopreventive activity, ubiquitous in plant food sources and a major bioflavonoid in the human diet, it also produces anti-inflammatory and anti-allergy effects mediated through the inhibition of the lipoxygenase and cyclooxygenase pathways, thereby preventing the production of pro-inflammatory mediators and Eriodictyol which is a flavonoid with anti-inflammatory and antioxidant activities. Because inflammation and oxidative stress play critical roles in the pathogenesis of diabetes mellitus, the present study was designed to explore whether Eriodictyol, Quercetin and Kaempferol have therapeutic potential for the treatment of common diseases. In this study we studied the anti-proliferative effect of the three flavonoids using RAW 246.7 cells as in vitro model, and observed a dose-dependent reduction in cell viability. We also evaluated the capacity of these flavonoids to inhibit, in vitro, the activity of selected enzymes to assess their potential use as anti-aging, neuroprotective and anti-diabetic agents.