Publicação
Human tooth-derived matrix granules for bone repair : a scoping review on the manufacturing
| Resumo: | Background: Human tooth-derived matrix (HTDM) granules are currently used for bone repair in implant dentistry. However, different guidelines for manufacturing HTDM granules are reported in literature. Objectives: The main objective of this work was to perform a scoping review of the manufacturing guidelines of human tooth-derived matrix granules for bone repair. Methods: A bibliographical search was carried out on both PubMed and Scopus with articles published in English language up to November 2024. The exclusion criteria involved review articles, pilot studies and short follow-up. Results: The selected studies reported different griding and disinfection procedures for manufacturing HTDM granules. The main procedure includes the removal of enamel and grinding the radicular dentin to acquire granules with size ranging from 300 up to 1200 μm. Then, the granules are disinfected in NaOH followed by immersion in EDTA resulting in a partial demineralization that exposes the collagen fibers and enlarging the dentin tubules. Growth factors and proteins were detected after the grinding and disinfection procedure. The content of the HTDM granules increased the viability of osteogenic cells after cell culture assays that indicates an enhanced stimuli for bone repair. Conclusions: Human tooth-derived matrix is a promising autologous material for bone repair considering their biological content. However, standard guidelines are required for improvement of surgical outcomes. |
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| Autores principais: | Marconi, Melvin Paul Mario |
| Assunto: | Dentin-derived matrix DBBM Bone healing Bone graft Matriz derivada de dentina Cicatrização óssea Enxerto ósseo |
| Ano: | 2025 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso embargado |
| Instituição associada: | Universidade Católica Portuguesa |
| Idioma: | inglês |
| Origem: | Veritati - Repositório Institucional da Universidade Católica Portuguesa |
| Resumo: | Background: Human tooth-derived matrix (HTDM) granules are currently used for bone repair in implant dentistry. However, different guidelines for manufacturing HTDM granules are reported in literature. Objectives: The main objective of this work was to perform a scoping review of the manufacturing guidelines of human tooth-derived matrix granules for bone repair. Methods: A bibliographical search was carried out on both PubMed and Scopus with articles published in English language up to November 2024. The exclusion criteria involved review articles, pilot studies and short follow-up. Results: The selected studies reported different griding and disinfection procedures for manufacturing HTDM granules. The main procedure includes the removal of enamel and grinding the radicular dentin to acquire granules with size ranging from 300 up to 1200 μm. Then, the granules are disinfected in NaOH followed by immersion in EDTA resulting in a partial demineralization that exposes the collagen fibers and enlarging the dentin tubules. Growth factors and proteins were detected after the grinding and disinfection procedure. The content of the HTDM granules increased the viability of osteogenic cells after cell culture assays that indicates an enhanced stimuli for bone repair. Conclusions: Human tooth-derived matrix is a promising autologous material for bone repair considering their biological content. However, standard guidelines are required for improvement of surgical outcomes. |
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