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Chitosan produced from Mucorales fungi using agroindustrial by-products and its efficacy to inhibit Colletotrichum species

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Detalhes bibliográficos
Resumo:This study evaluated corn steep liquor (CSL) and papaya peel juice (PPJ) in mixture as substrates for thecultivation (96 h, 28◦C, pH 5.6, 150 rpm) of Mucorales fungi for chitosan production, and determinedthe growth-inhibitory effect of the fungal chitosan (FuCS) obtained under optimized conditions againstphytopathogenic Colletotrichum species. All Mucorales fungi tested were capable of growing in CSL-PPJmedium, showing FuCS production in the range of 5.02 (Fennelomyces heterothalicus SIS 28) − 15.63 mg/g(Cunninghamella elegans SIS 41). Highest FuCS production (37.25 mg/g) was achieved when C. eleganswas cultivated in medium containing 9.43% CSL and 42.5% PPJ. FuCS obtained under these conditionsshowed a deacetylation degree of 86%, viscosity of 120 cP and molecular weight of 4.08 × 104g/mol.FuCS at 5000, 7500 and 10,000 ppm inhibited the growth of all Colletotrichum species tested. FuCS alsoinduced alterations in the morphology of C. fructicola hyphae. CSL-PPJ mixtures are suitable substratesfor the cultivation of Mucorales fungi for FuCS production. Chitosan from C. elegans cultivated in CSL-PPJmedium is effective in inhibiting phytopathogenic Colletotrichum species.
Autores principais:Bergera, Lúcia Raquel Ramos
Outros Autores:Stamford, Thayza Christina Montenegro; Oliveira, Kataryne Árabe Rimáde; Pessoa, Adjanede Miranda Pereira; Lima, Marcos Antonio Barbosa de; Pintado, Maria Manuela Estevez; Câmara, Marcos Paz Saraiva; Franco, Lucianade Oliveira; Souza, Marciane Magnania Evandro Leite de; Souza, Evandro Leite de
Assunto:Agroindustrial by-products Fungal chitosan Antifungal effects
Ano:2018
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso restrito
Instituição associada:Universidade Católica Portuguesa
Idioma:inglês
Origem:Veritati - Repositório Institucional da Universidade Católica Portuguesa
Descrição
Resumo:This study evaluated corn steep liquor (CSL) and papaya peel juice (PPJ) in mixture as substrates for thecultivation (96 h, 28◦C, pH 5.6, 150 rpm) of Mucorales fungi for chitosan production, and determinedthe growth-inhibitory effect of the fungal chitosan (FuCS) obtained under optimized conditions againstphytopathogenic Colletotrichum species. All Mucorales fungi tested were capable of growing in CSL-PPJmedium, showing FuCS production in the range of 5.02 (Fennelomyces heterothalicus SIS 28) − 15.63 mg/g(Cunninghamella elegans SIS 41). Highest FuCS production (37.25 mg/g) was achieved when C. eleganswas cultivated in medium containing 9.43% CSL and 42.5% PPJ. FuCS obtained under these conditionsshowed a deacetylation degree of 86%, viscosity of 120 cP and molecular weight of 4.08 × 104g/mol.FuCS at 5000, 7500 and 10,000 ppm inhibited the growth of all Colletotrichum species tested. FuCS alsoinduced alterations in the morphology of C. fructicola hyphae. CSL-PPJ mixtures are suitable substratesfor the cultivation of Mucorales fungi for FuCS production. Chitosan from C. elegans cultivated in CSL-PPJmedium is effective in inhibiting phytopathogenic Colletotrichum species.