Autor(es): Roriz, Custódio Lobo ; Heleno, Sandrina A. ; Alves, Maria José ; Oliveira, Beatriz ; Pinela, José ; Dias, Maria Inês ; Calhelha, Ricardo C. ; Morales, Patricia ; Ferreira, Isabel C.F.R. ; Barros, Lillian
Data: 2022
Identificador Persistente: http://hdl.handle.net/10198/24153
Origem: Biblioteca Digital do IPB
Assunto(s): Hylocereus costaricensis; Bioactive compounds; Betacyanins; Natural colorants; Ultrasound-assisted extraction; Process optimization
Hylocereus costaricensis peel contains large amounts of betacyanins and can be exploited as a source of natural colorants. This work aimed the chemical characterization and evaluation of bioactive properties of this byproduct and the optimization of the ultrasound-assisted extraction (UAE) of betacyanins using the response surface methodology (RSM). Oxalic and malic acids and traces of fumaric acid were detected, as well as the four tocopherol isoforms, predominantly γ-tocopherol. Four betacyanins were identified and used as response criteria for UAE optimization, namely phyllocactin, isobetanin, isophyllocactin, and betanin. Sample processing at 487 W for 38 min result in the maximum betacyanin content (36 ± 1 mg/g dw). The peel extract inhibit the oxidative haemolysis, with IC50 values of 255 and 381 μg/mL for Δt of 60 and 120 min, respectively, and the growth of pathogenic bacteria, with minimum inhibitory concentrations ranging from 5 to 20 mg/mL. Furthermore, no toxicity was observed for normal cells.
