Generation and characterization of a human iPS cell line from a patient-related control to study disease mechanisms associated with DAND5 p.R152H alteration

• Author(s): Pars, Selin ; Cristo, Fernando ; Inácio, José M. ; Rosas, Graça ; Carreira, Isabel Marques ; Melo, Joana Barbosa ; Mendes, Patrícia ; Martins, Duarte Saraiva ; Almeida, Luís Pereira de ; Maio, José ; Anjos, Rui ; Belo, José A.
• Date: 2018
• Persistent ID: https://hdl.handle.net/10316/108062
• Origin: Estudo Geral - Universidade de Coimbra
• Project/scholarship: info:eu-repo/grantAgreement/other/Multi/04462/other
• Subject(s): Cell Line; Humans; Male; Cellular Reprogramming Techniques; Heart Diseases; Induced Pluripotent Stem Cells; Intercellular Signaling Peptides and Proteins
• Document Type: Journal article
• Language: English

Description

A DAND5-control human iPSC line was generated from the urinary cells of a phenotypically normal donor. Exfoliated renal epithelial (RE) cells were collected and reprogrammed into iPSCs using Sendai virus reprogramming system. The pluripotency, in vitro differentiation potential, karyotype stability, and the transgene-free status of generated iPSC line were analyzed and confirmed. This cell line can be exploited as a control iPSC line to better understand the mechanisms involved in DAND5-associated cardiac disease.