Author(s):
Ventura, Célia ; Marques, Catarina ; Cadete, João ; Vilar, Madalena ; Pedrosa, Jorge F. S. ; Pinto, Fátima ; Fernandes, Susete Nogueira ; da Rosa, Rafaela Raupp ; Godinho, Maria Helena ; Ferreira, Paulo J. T. ; Louro, Henriqueta ; Silva, Maria João
Date: 2022
Persistent ID: https://hdl.handle.net/10316/99908
Origin: Estudo Geral - Universidade de Coimbra
Project/scholarship:
info:eu-repo/grantAgreement/other/| PTDC/SAU-PUB/other;
info:eu-repo/grantAgreement/other///other;
info:eu-repo/grantAgreement/other/PTDC/CTM-REF/other;
info:eu-repo/grantAgreement/other///other;
Subject(s): nanofibrillated cellulose; nanocrystalline cellulose; nanotoxicology; cytotoxicity; genotoxicity; micronucleus assay
Description
Background: Nanocellulose is an innovative engineered nanomaterial with an enormous potential for use in a wide array of industrial and biomedical applications and with fast growing economic value. The expanding production of nanocellulose is leading to an increased human exposure, raising concerns about their potential health effects. This study was aimed at assessing the potential toxic and genotoxic effects of different nanocelluloses in two mammalian cell lines; (2) Methods: Two micro/nanocelluloses, produced with a TEMPO oxidation pre-treatment (CNFs) and an enzymatic pre-treatment (CMFs), and cellulose nanocrystals (CNCs) were tested in osteoblastic-like human cells (MG-63) and Chinese hamster lung fibroblasts (V79) using the MTT and clonogenic assays to analyse cytotoxicity, and the micronucleus assay to test genotoxicity; (3) Results: cytotoxicity was observed by the clonogenic assay in V79 cells, particularly for CNCs, but not by the MTT assay; CNF induced micronuclei in both cell lines and nucleoplasmic bridges in MG-63 cells; CMF and CNC induced micronuclei and nucleoplasmic bridges in MG-63 cells, but not in V79 cells; (4) Conclusions: All nanocelluloses revealed cytotoxicity and genotoxicity, although at different concentrations, that may be related to their physicochemical differences and availability for cell uptake, and to differences in the DNA damage response of the cell model.
The research was funded by the Portuguese Foundation for Science and Technology (FCT/MCTES), through national funds (PIDDAC) under the project ToxApp4NanoCELFI (PTDC/SAU-PUB/32587/2017). The following funding is also acknowledged: POCI-01-0145-FEDER-007688 (UIDB/50025/2020-2023); NanoCell2SEC (PTDC/CTM-REF/30529/2017); ToxOmics (UIDP/00009/2020; UIDB/00009/2020); CIEPQPF (UIDB/00102/2020); European Topology Interdisciplinary Action (EUTOPIA CA17139); Inpactus Project—innovative products and technologies from eucalyptus (Project N.◦ 21874 funded by Portugal 2020 through ERDF in the frame of COMPETE 2020 n.º 246/AXIS II/2017).
