Autor(es): Brandão, Geane Peroni ; Melo, Maria Norma ; Caetano, Bráulia Costa ; Carneiro, Cláudia Martins ; Silva, Letícia de Azevedo ; Vitor, Ricardo Wagner de Almeida
Data: 2012
Origem: Oasisbr
Assunto(s): Experimental toxoplasmosis; Re-infection; Recombinant strains
This work reports results of re-infection of BALB/c and C57BL/6 mice with different recombinant strains of Toxoplasma gondii. Mice were prime-infected with the non-virulent D8 strain and challenged with virulent strains. PCR–RFLP of cS10-A6 genetic marker of T. gondii demonstrated that BALB/c mice were reinfected with the EGS strain, while C57BL/6 mice were re-infected with the EGS and CH3 strains. Levels of IFN-c and IL-10 after D8 prime-infection were lower in C57BL/6 than in BALB/c mice. Brain inflammation after D8 prime-infection was more intense in C57BL/6 than in BALB/c mice. It was shown that reinfection depends on mice lineage and genotype of the strain used in the challenge.
